Localization and changes of diamine oxidase during cardiopulmonary bypass in rabbits.
Localization and changes of diamine oxidase during cardiopulmonary bypass in rabbits.
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DOI：
10.1016/j.jss.2005.10.004
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发表时间：
2006-03
期刊：
影响因子：
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通讯作者：
N. Tsunooka;K. Maeyama;H. Nakagawa;T. Doi;Atsushi Horiuchi;K. Miyauchi;Yuji Watanabe;H. Imagawa;K. Kawachi
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文献类型：
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作者：
N. Tsunooka;K. Maeyama;H. Nakagawa;T. Doi;Atsushi Horiuchi;K. Miyauchi;Yuji Watanabe;H. Imagawa;K. Kawachi
背景我们既往在临床体外循环过程中观察到血清二胺氧化酶活性升高，提示小肠粘膜缺血，继而发生细菌移位。材料与方法在7只雌性兔中进行1小时的体外循环，采用免疫组织化学方法分析二胺氧化酶的定位，并测定其在体外循环（CPB）前后血清和腹部器官中的活性。结果术前，二胺氧化酶活性和免疫反应性位于小肠的高位，位于绒毛尖端。 CPB后血清活性显着增加，而小肠二胺氧化酶随着粘膜损伤而降低。结论在该模型中，血清二胺氧化酶活性似乎反映了CPB引起的肠粘膜损伤。
BACKGROUNDWe previously observed increased serum diamine oxidase activity during clinical cardiopulmonary bypass, indicating small intestinal mucosal ischemia followed by bacterial translocation.MATERIALS AND METHODSIn seven female rabbits undergoing cardiopulmonary bypass for 1 h, we analyzed the localization of diamine oxidase immunohistochemically, and measured its activity in serum and abdominal organs before and after cardiopulmonary bypass (CPB).RESULTSPreoperatively, diamine oxidase activity and immunoreactivity were high in the small intestine, localized to villus tips. Serum activity increased significantly after CPB, whereas small intestinal diamine oxidase decreased with mucosal injury.CONCLUSIONSIn this model serum diamine oxidase activity appeared to reflect CPB induced intestinal mucosal injury.