A Compact Head-Mounted Endoscope for In Vivo Calcium Imaging in Freely Behaving Mice.
A Compact Head-Mounted Endoscope for In Vivo Calcium Imaging in Freely Behaving Mice.
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DOI：
10.1002/cpns.51
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发表时间：
2018-07-01
影响因子：
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通讯作者：
Josselyn, Sheena A
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文献类型：
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作者：
Jacob, Alexander D;Ramsaran, Adam I;Josselyn, Sheena A
微型荧光显微镜成像钙瞬变是一个很有前途的工具，研究行为和群体水平的神经元活动之间的关系在啮齿动物。然而，商业上可获得的微型显微镜可能是昂贵的，并且因为它们是封闭源，可能不容易基于特定的实验要求进行修改。在这里，我们描述了如何建立和使用一个低成本的紧凑型头戴式内窥镜（CHEndoscope）系统在体内钙成像。CHEndoscope使用植入的梯度折射率透镜沿着以及遗传编码的钙指示剂GCaMP 6，在清醒行为的小鼠中同时对数百个神经元的钙瞬变成像。该系统价格实惠，开源，灵活，允许根据特定的实验进行修改。本文详细描述了使用CHEndoscope系统的组装、手术植入、数据收集和钙信号处理。这个开放框架的目的是为神经科学研究社区提供一套可访问的小型化钙成像工具。John Wiley & Sons，Inc.
Miniaturized fluorescence microscopes for imaging calcium transients are a promising tool for investigating the relationship between behavior and population-level neuronal activity in rodents. However, commercially available miniature microscopes may be costly and, because they are closed source, may not be easily modified based on particular experimental requirements. Here, we describe how to build and use a low-cost compact head-mounted endoscope (CHEndoscope) system for in vivo calcium imaging. The CHEndoscope uses an implanted gradient index lens along with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice. This system is affordable, open source, and flexible, permitting modification depending on the particular experiment. This article describes in detail the assembly, surgical implantation, data collection, and processing of calcium signals using the CHEndoscope system. The aim of this open framework is to provide an accessible set of miniaturized calcium imaging tools for the neuroscience research community. © 2018 by John Wiley & Sons, Inc.