Expression of l-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in liver tumor lesions of rat models
Expression of l-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in liver tumor lesions of rat models
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DOI：
10.1002/jso.1165
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发表时间：
2001-12-01
影响因子：
2.5
通讯作者：
Kanai, Y
中科院分区：
文献类型：
--
作者：
Ohkame, H;Masuda, H;Kanai, Y
背景和目标：氨基酸转运蛋白在细胞营养供应和细胞增殖中起着重要作用。在这项研究中，我们研究是否LAT 1和4F 2 hc是密切相关的肿瘤growth.Methods：大鼠结肠癌细胞（RCN-9）注入12只雄性大鼠（近交系F344/DuCrj）的脾脏。在每只大鼠中，用抗LAT 1和抗4F 2 hc抗体对包括肿瘤病变的肝脏样品进行免疫染色。结果：12例肝脏标本共观察到68个肿瘤结节。在68个肿瘤结节中，36个结节（52.9%）显示LAT 1阳性染色，32个（47.1%）显示LAT 1阴性染色。然而，在非肿瘤区域几乎检测不到LAT 1的表达。在4F 2 hc表达方面，56个结节（82.4%）4F 2 hc阳性，12个（17.6%）4F 2 hc阴性。4F 2 hc在非肿瘤区的表达与肿瘤区的表达基本一致。LAT 1阳性和4F 2 hc阳性组（n = 31）的平均肿瘤大小为0.845 +/- 0.232 mm（2），与LAT 1阴性组和4F 2 hc阴性组（n=7）相比，差异有显著性（P <0.05）。（0.090 ± 0.028 mm（2））或LAT 1阳性和4F 2 hc阴性组（n = 5）（0.097 ± 0.025 mm（2））（P = 0.0017，P = 0.007）。我们认为，LAT 1可能与4F 2 hc协同作用，增强其促肿瘤生长的能力。外科肿瘤学杂志2001;78：265-272. (C)2001 Wiley-Liss，Inc.
Background and Objectives: It has been said that amino acid transporters play an important role in supplying nutrition to cells and for cell proliferation. In this study, we examined whether LAT1 and 4F2hc are closely related to tumor growth.Methods: Rat colon cancer cells (RCN-9) were injected into the spleen of 12 male rats (inbred F344/DuCrj). In each rat, liver samples including tumor lesions were immunostained with anti-LAT1 and anti-4F2hc antibodies. The staining area of LAT1 and 4F2hc tumor lesions was calculated by computer analysis.Results: Sixty-eight tumor nodules were observed in 12 livers. Out of the 68 tumor nodules, 36 nodules (52.9%) indicated a positive staining of LAT1 and 32 (47.1%) had a negative staining of LAT1. However, the LAT1 expression was scarcely detected in non-tumor areas. In terms of the 4F2hc expression, there were 56 nodules (82.4%) with 4F2hc positive and 12 (17.6) with 4F2hc negative. In addition, the expression of 4F2hc in nontumor areas was almost the same as the expression of 4F2hc in tumor lesions. The average tumor size of the group with LAT1 positive and 4F2hc positive (n = 31) was 0.845 +/- 0.232 mm(2), which was significantly larger than that of the group with LAT1 negative and 4F2hc negative group (n=7) (0.090 +/- 0.028 mm(2)) or the group with LAT1 positive and 4F2hc negative (n = 5) (0.097 +/- 0.025 mm(2)), respectively (P = 0.0017, P = 0.007).Conclusion: LAT1 was related to tumor growth. We think that LAT1 can possibly enhance its ability to promote tumor growth in cooperation with 4F2hc. J. Surg. Oncol. 2001;78:265-272. (C) 2001 Wiley-Liss, Inc.