A Quick and Safe Method for Fungal DNA Extraction
A Quick and Safe Method for Fungal DNA Extraction
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DOI：
10.5423/ppj.2009.25.1.108
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发表时间：
2009-03-01
影响因子：
2.3
通讯作者：
Lee, Yong-Hwan
中科院分区：
文献类型：
--
作者：
Chi, Myoung-Hwan;Park, Sook-Young;Lee, Yong-Hwan
基于DNA的研究，包括克隆和基因分型，已成为真菌研究实验室的常规。然而，从真菌组织中制备高质量的DNA需要大量的时间和劳动，并且通常是高通量实验的限制步骤。本研究建立了一种快速、安全的真菌DNA提取方法。QS方法的时间效率和安全性通过使用平板生长的菌丝体作为起始材料，通过消除酚-氯仿提取程序，并通过部署一个简单的电动研磨机来实现。这种QS方法不仅适用于广泛的微生物真核生物，包括真真菌和卵菌，而且适用于地衣和植物。
DNA-based studies, including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating phenol-chloroform extraction procedures, and by deploying a simple electric grinder. This QS method is applicable not only to a broad range of microbial eukaryotes, including true fungi and oomycetes, but also to lichens and plants.