The effect of serum on monolayer cell culture of mammalian articular chondrocytes.
The effect of serum on monolayer cell culture of mammalian articular chondrocytes.
复制标题
血清对哺乳动物关节软骨细胞单层细胞培养的影响。
DOI：
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发表时间：
1980
影响因子：
2.9
通讯作者：
L. Sokoloff
中科院分区：
文献类型：
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作者：
Y. C. Choi;G. M. Morris;F. Lee;L. Sokoloff
在单层培养中，6种血清支持兔、人和狗关节软骨细胞生长的能力差异很大。在大多数情况下，当血清浓度从10%增加到30%时，细胞颗粒的DNA含量直接增加。在一些血清中发现了抑制和促进生长作用的迹象。血清浓度升高对兔软骨细胞增殖的刺激作用伴随着放射性硫酸盐掺入和细胞蛋白含量的降低。来自某一物种的软骨细胞的反应与其同源血清之间没有一致的关系。然而，人血清可大大促进人软骨细胞的生长，只要用胎牛血清启动可克服同种血清对细胞与培养瓶初始附着的干扰。
Large variations were found in the ability of six species of sera to support growth of rabbit, human and dog articular chondrocytes in monolayer culture. In most instances the DNA content of the cell pellets increased directly as the serum concentration rose from 10 to 30%. Indications of inhibitory as well as growth-promoting actions were found in some sera. Stimulation of rabbit chondrocyte proliferation by increasing concentrations of serum was accompanied by a reduction of radiosulfate incorporation and cell protein content. There was no consistent relation between the response of chondrocytes from a given species and its homologous serum. However, the growth of human chondrocytes was greatly potentiated by human serum provided that interference with initial attachment of the cells to the culture flask by homologous serum was overcome by priming with fetal calf serum.
DOI：
10.1002/9780470720974.ch7
发表时间：
1985
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
R. Ross;D. Bowen-Pope;E. Raines
通讯作者：
R. Ross;D. Bowen-Pope;E. Raines