TRANSFORMING GROWTH FACTOR-BETA STIMULATES THE EXPRESSION OF ENDOTHELIN MESSENGER-RNA BY VASCULAR ENDOTHELIAL-CELLS
TRANSFORMING GROWTH FACTOR-BETA STIMULATES THE EXPRESSION OF ENDOTHELIN MESSENGER-RNA BY VASCULAR ENDOTHELIAL-CELLS
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DOI：
10.1016/0006-291x(89)92270-5
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发表时间：
1989-03-31
影响因子：
3.1
通讯作者：
YAZAKI, Y
中科院分区：
文献类型：
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作者：
KURIHARA, H;YOSHIZUMI, M;YAZAKI, Y
内皮素是由血管内皮细胞产生的一种有效的血管收缩肽。用10-300 pM TGF-β 1孵育去除血清的汇合的猪主动脉内皮细胞，导致内皮素mRNA水平的几倍增加，峰值时间为2小时。酶联免疫吸附测定揭示了内皮细胞条件培养基中内皮素的水平也因TGF-β 1而增加。这些结果表明，由活化的血小板分泌的TGF-β 1不仅参与伤口愈合，而且通过刺激内皮细胞中内皮素的产生而参与局部血管张力的调节。
Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-.beta.1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endolthelial cell conditioned media was also increased by TGF-.beta.1. These results suggest that TGF-.beta.1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.