Identification and distribution of Parkin in rat brain
Identification and distribution of Parkin in rat brain
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DOI：
10.1097/00001756-199911080-00025
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发表时间：
1999-11-08
期刊：
影响因子：
1.7
通讯作者：
Torres, G
中科院分区：
文献类型：
--
作者：
Horowitz, JM;Myers, J;Torres, G
Parkin基因编码蛋白Parkin的氨基酸序列内的突变似乎引发黑质多巴胺能神经元的变性。在此，检查大鼠体内Parkin的存在和解剖分布。组织匀浆的免疫印迹分析显示在50和44 kDa处有两个主要条带。在脑内，在基底神经节（包括黑质和尾壳核）中鉴定出含帕金森病的神经元。帕金在中缝核中被观察到，中缝核和黑质一样，与单胺能编码神经元密切相关。此外，在层状结构如皮质和海马中检测到帕金;在小脑中也观察到大量的帕金免疫反应性神经元，因此帕金广泛分布于与帕金森病病理学有关的脑通路中。疾病NeuroReport port 10：3393 - 3397（C）1999 Lippincott威廉姆斯和威尔金斯。
MUTATIONS within the amino acid sequence of Parkin, the encoded protein of the parkin gene, appear to trigger the degeneration of dopaminergic neurons in the substantia nigra. Here, the presence and anatomical distribution of Parkin within-the rat was examined. Immunoblot analysis of tissue homogenates showed two major bands at 50 and 44 kDa. Within the brain, Parkin-containing neurons were identified in the basal ganglia, including the substantia nigra and caudate-putamen. Parkin was visualized in the raphe nucleus, which as in the substantia nigra, was closely localized to monoaminergic-encoding neurons. In addition,Parkin was detected in laminar structures such as the cortex and hippocampus; a substantial number of Parkin-immunoreactive neurons was seen in the cerebellum as well, Parkin therefore is widely distributed in brain pathways implicated in the pathology of Parkinson's. disease. NeuroReport port 10:3393-3397 (C) 1999 Lippincott Williams & Wilkins.