Fungal and Bacterial Contamination on Indoor Surfaces of a Hospital in Mexico
Fungal and Bacterial Contamination on Indoor Surfaces of a Hospital in Mexico
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DOI：
10.5812/jjm.2625
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发表时间：
2012-01-01
影响因子：
0.6
通讯作者：
Elind Arroyo-Helguera, Omar
中科院分区：
文献类型：
--
作者：
Pastor Garcia-Cruz, Cesar;Najera Aguilar, Maria Josefina;Elind Arroyo-Helguera, Omar
背景资料：在住院期间获得的医院感染取决于微生物的特性，当环境被污染时，获得这些微生物的风险很高。交叉传播的微生物污染的表面和卫生保健工作者的手被认为是医院感染的传播的主要途径，在许多墨西哥医院，有关室内表面的微生物和真菌污染物的信息是不够的。扶手床，洗手池，医疗桌，以及来自急诊室，重症监护室，吸入治疗，口腔科和儿科区域的医务人员的手。这些样品是在2010年8月至12月的6个月期间采集的。用棉签从预定义的表面采集表面拭子样本，用无菌盐水预润湿，接种在沙氏葡萄糖或选择性伊红-亚甲基蓝琼脂平板上，并在28 ℃或37 ℃下培养1至7天，一式三份，以分离细菌和真菌污染物。结果表明，主要致病菌为克雷伯氏菌、革兰氏阴性菌和真菌。假单胞菌和大肠杆菌（E coli spp.）真菌的优势属为枝孢属（Cladosporium spp.）犬小孢子菌，曲霉属和青霉属（Penicillium spp.）这项研究表明，医院的所有区域都有致病真菌和细菌，这表明这些是从医护人员手中交叉感染到患者的潜在来源。版权所有（C）2012 Kowsar Corp.
Background: Nosocomial infections acquired during hospitalization depend on the characteristics of the microorganisms, with a high risk of these being acquired when the environment is contaminated. Cross-transmission of microorganisms by contaminated surfaces and the hands of health care workers are considered to be the main route of the spread of nosocomial infections and in many Mexican hospitals, information about microbial and fungi contaminants on indoor surfaces is insufficient.Objectives: In this study, we explore the bacterial and fungal genera present on surfaces taken from; armrest beds, wash sinks, medical tables, and the hands of medical staff from emergency, intensive care unit, inhalation therapy, stomatology and pediatric areas.Materials and Methods: The present study was conducted in a hospital located in Xalapa City in Mexico; the samples were taken during a 6 month period from August to December, 2010. Surface swab specimens were collected from predefined surfaces with cotton tipped applicators, pre-moistened with sterile saline and inoculated on Sabouraud dextrose or selective Eosin-Methylene Blue agar plates and incubated for 1 to 7 days at 28 degrees C or 37 degrees C in three triplications in order to isolate bacterial and fungal contaminants.Results: We isolated and identified gram-negative bacteria and fungi and the results showed that the predominant bacteria were; Klebsiella spp. Pseudomonas spp. and E coli spp. The predominant genera of fungi were; Cladosporium spp. Microsporum canis., Aspergillus spp. and Penicillium spp.Conclusions: This study showed that all areas of the hospital have pathogenic fungi and bacteria, indicating that these are a potential source of cross-infection from the hands of health care workers to their patients. Copyright (C) 2012 Kowsar Corp. All rights reserved.