Direct freeform fabrication of seeded hydrogels in arbitrary geometries
Direct freeform fabrication of seeded hydrogels in arbitrary geometries
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DOI：
10.1089/ten.2006.12.1325
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发表时间：
2006-05-01
影响因子：
--
通讯作者：
Bonassar, Lawrence J.
中科院分区：
文献类型：
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作者：
Cohen, Daniel L.;Malone, Evan;Bonassar, Lawrence J.
组织工程中的一个主要挑战是产生具有模拟天然组织的结构的细胞接种植入物，无论是在解剖几何形状还是组织内细胞分布。通过将注射成型组织工程的优势与固体自由成型制造（SFF）的优势相结合，在没有定制工具的情况下制造三维（3-D）预接种植入物，从而能够有效地生产患者特定的植入物。SFF技术的结合还使得能够制造几何形状复杂的多材料植入物，其具有空间异质性，否则将具有生产挑战性。利用定制的机器人SFF平台和凝胶沉积工具，使用藻酸盐水凝胶与硫酸钙作为交联剂来生产任意几何形状的预接种活体植入物。确定该过程无菌，且在94 +/-5%时存活。发现GAG和羟脯氨酸的产生与使用相同材料和不同成型方法制造的其他植入物相似。通过使用印刷平台作为计算机化测量机器（CMM）来量化该过程的几何保真度;发现印刷样品在z维度上的RMS表面粗糙度为0.16 +/-0.02 mm。
A major challenge in tissue engineering is the generation of cell-seeded implants with structures that mimic native tissue, both in anatomic geometries and intratissue cell distributions. By combining the strengths of injection molding tissue engineering with those of solid freeform fabrication (SFF), three-dimensional (3-D) pre-seeded implants were fabricated without custom-tooling, enabling efficient production of patient-specific implants. The incorporation of SFF technology also enabled the fabrication of geometrically complex, multiple-material implants with spatially heterogeneous properties that would otherwise be challenging to produce. Utilizing a custom-built robotic SFF platform and gel deposition tools, alginate hydrogel was used with calcium sulfate as a crosslinking agent to produce pre-seeded living implants of arbitrary geometries. The process was determined to be sterile and viable at 94 +/- 5%. The GAG and hydroxyproline production was found to be similar to that of other implants fabricated using the same materials with different shaping methods. The geometric fidelity of the process was quantified by using the printing platform as a computerized measurement machine (CMM); the RMS surface roughness of printed samples in the z-dimension was found to be 0.16 +/- 0.02 mm.