Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown
Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown
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DOI：
10.1021/ac071162h
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发表时间：
2007-09-01
影响因子：
7.4
通讯作者：
Han, Jongyoon
中科院分区：
文献类型：
--
作者：
Lee, Jeong Hoon;Chung, Seok;Han, Jongyoon
Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nano-fluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of beta-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 104 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).
