Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation.
Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation.
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DOI：
10.3969/j.issn.1673-5374.2012.19.002
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发表时间：
2012-07-05
影响因子：
6.1
通讯作者：
Lu K
中科院分区：
文献类型：
--
作者：
Lu J;Li D;Lu K
本研究探讨了成纤维细胞生长因子-8及其潜在受体成纤维细胞生长因子-3在体内成年大鼠脑和体外神经干/祖细胞分化过程中在神经细胞中的分布和定位。采用免疫组织化学方法检测成纤维细胞生长因子-8在成年大鼠脑内的分布。采用免疫荧光法检测神经干细胞/祖细胞体外分化过程中成纤维细胞生长因子-8和成纤维细胞生长因子受体-3在细胞中的定位。采用流式细胞术和免疫荧光法观察抗成纤维细胞生长因子-8抗体对体外培养的神经干/祖细胞分化扩增的影响。本研究结果证实成纤维细胞生长因子-8主要分布于成人中脑，即黑质、致密部分、背层、黑质和网状部分，而未在包括尾状壳核和纹状体的前脑中检测到。在成年大鼠脑脾后部位得到不同寻常的结果。通过免疫组织化学和免疫荧光分析，我们发现成纤维细胞生长因子-8和成纤维细胞生长因子受体-3分布在神经细胞的细胞膜和细胞质中。我们认为成纤维细胞生长因子-8和成纤维细胞生长因子受体-3在神经细胞中的分布与成纤维细胞生长因子-8这一分泌因子的特征相对应。在培养物中加入抗成纤维细胞生长因子-8抗体，显著影响神经干细胞/祖细胞的增殖和分化率。相比之下，在分化培养基中添加重组成纤维细胞生长因子-8促进了神经干/祖细胞的分化，并增加了多巴胺能神经元和总神经元的最终产量。我们的研究可能有助于描述成纤维细胞生长因子-8在脑活动和神经干/祖细胞分化中的重要作用。
The present study explored the distribution and localization of fibroblast growth factor-8 and its potential receptor, fibroblast growth factor receptor-3, in adult rat brain in vivo and in nerve cells during differentiation of neural stem/progenitor cells in vitro. Immunohistochemistry was used to examine the distribution of fibroblast growth factor-8 in adult rat brain in vivo. Localization of fibroblast growth factor-8 and fibroblast growth factor receptor-3 in cells during neural stem/progenitor cell differentiation in vitro was detected by immunofluorescence. Flow cytometry and immunofluorescence were used to evaluate the effect of an anti-fibroblast growth factor-8 antibody on neural stem/progenitor cell differentiation and expansion in vitro. Results from this study confirmed that fibroblast growth factor-8 was mainly distributed in adult midbrain, namely the substantia nigra, compact part, dorsal tier, substantia nigra and reticular part, but was not detected in the forebrain comprising the caudate putamen and striatum. Unusual results were obtained in retrosplenial locations of adult rat brain. We found that fibroblast growth factor-8 and fibroblast growth factor receptor-3 were distributed on the cell membrane and in the cytoplasm of nerve cells using immunohistochemistry and immunofluorescence analyses. We considered that the distribution of fibroblast growth factor-8 and fibroblast growth factor receptor-3 in neural cells corresponded to the characteristics of fibroblast growth factor-8, a secretory factor. Addition of an anti-fibroblast growth factor-8 antibody to cultures significantly affected the rate of expansion and differentiation of neural stem/progenitor cells. In contrast, addition of recombinant fibroblast growth factor-8 to differentiation medium promoted neural stem/progenitor cell differentiation and increased the final yields of dopaminergic neurons and total neurons. Our study may help delineate the important roles of fibroblast growth factor-8 in brain activities and neural stem/progenitor cell differentiation.
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