Two Species of Canine Babesia in Australia: Detection and Characterization by PCR
Two Species of Canine Babesia in Australia: Detection and Characterization by PCR
复制标题
澳大利亚两种犬巴贝斯虫：PCR 检测和表征
作者：
R. Jefferies;U. Ryan;C. Muhlnickel;P. Irwin
血液原生动物犬巴贝斯虫在澳大利亚已经被发现多年，最近又发现了第二个较小的物种。18S小亚基核糖体RNA(RRNA)基因部分区域的扩增和测序首次实现了对澳大利亚大小犬巴贝斯虫的检测和鉴定。来自澳大利亚北部的分离株在基因特征上与犬巴贝斯虫Vogeli有99%的同源性，证实了之前关于澳大利亚特有的犬巴贝斯虫亚种的猜测。从澳大利亚东南部获得的分离物中扩增出的部分18S rRNA基因序列与吉氏巴贝斯虫(Babesia Gibsoni)在基因上相同，后者是澳大利亚以前未知的物种。聚合酶链式反应被证明是巴贝斯虫特异的，并且具有很高的灵敏度，在大约0.0000027%的寄生虫血症中检测到脱氧核糖核酸。这项研究还首次报道了利用聚合酶链式反应检测和鉴定血缘管圆线虫体内犬芽胞杆菌DNA的情况。
The haemoprotozoan Babesia canis has been recognized in Australia for many years, and a second, smaller species has recently been discovered. Amplification and sequencing of a partial region of the 18S small subunit ribosomal RNA (rRNA) gene enabled detection and characterization of the large and small canine babesiae of Australia for the first time. Isolates from northern Australia were genetically characterized to be 99% homologous to Babesia canis vogeli, confirming previous speculation about the subspecies of B. canis endemic to Australia. The partial 18S rRNA gene sequence amplified from isolates obtained in southeastern Australia was genetically identical to Babesia gibsoni, a species not previously known in Australia. The polymerase chain reaction (PCR) used was shown to be specific to Babesia and had a high sensitivity, detecting DNA at a parasitemia of approximately 0.0000027%. This study also reports the first known detection and characterization of B. canis DNA in Rhipicephalus sanguineus ticks using PCR.