Terminal lymphatics:: The potential "lethal corner" in the distribution of tissue pO2
Terminal lymphatics:: The potential "lethal corner" in the distribution of tissue pO2
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DOI：
10.1089/lrb.2007.5303
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发表时间：
2007-01-01
影响因子：
1.4
通讯作者：
Intaglietta, Marcos
中科院分区：
文献类型：
--
作者：
Hangai-Hoger, Nanae;Tsai, Amy G.;Intaglietta, Marcos
背景资料：末端淋巴液是离氧气供应最远的隔室，因此由于组织和淋巴管壁的氧气消耗，末端淋巴液应该在组织中呈现最低的pO（2）。方法和结果：在仓鼠室窗模型的组织、淋巴微血管、小动脉和小静脉中测定pO（2）的分布，其在没有麻醉的情况下用从环境中分离的组织进行研究。用磷光氧猝灭法测定淋巴液pO（2）。小终末淋巴液pO（2）为18.4 +/- 2.6 mmHg，集合淋巴管为18.0 +/- 2.4 mmHg。组织pO（2）平均值为24.6 +/- 2.7 mmHg。组织和淋巴管内pO（2）之间的显著差异部分是由于淋巴管壁存在氧梯度，其范围从终末淋巴管的3.7 +/- 1.3 mmHg到集合淋巴管的6.0 +/- 1.2 mmHg。这种梯度被认为是由于氧的消耗由细胞成分的lymphatic wall.Conclusion：血管壁梯度增加，发现在收集淋巴管被调和的发现，这些微淋巴管往往是连续的小动脉，而终端淋巴管分散在组织中。这些发现表明，终末淋巴呈现组织中最低的氧张力，因此是微血管氧供应受限时发生缺氧的危险部位。
Background: Terminal lymphatic fluid is the compartment furthest removed from the oxygen supply, and therefore should present the lowest pO(2) in the tissue due to oxygen consumption by the tissue and the lymphatic vessel wall.Methods and Results: The distribution Of pO(2) was determined in the tissue, the lymphatic microvessels, and arterioles and venules of the hamster chamber window model, which is studied without anesthesia with the tissue isolated from the environment. Lymphatic fluid pO(2) was measured with the phosphorescence oxygen quenching method. Small terminal lymphatic fluid pO(2) was 18.4 +/- 2.6 mmHg, and 18.0 +/- 2.4 mmHg in collecting lymphatics. Tissue pO(2) averaged 24.6 +/- 2.7 mmHg. The significant difference between tissue and intralymphatic pO(2) was due in part to the presence of an oxygen gradient across the lymphatic wall, which ranged from 3.7 +/- 1.3 mmHg for terminal lymphatics, to 6.0 +/- 1.2 mmHg for collecting lymphatics. This gradient is assumed to be due to the oxygen consumption by the cellular component of the lymphatic wall.Conclusion: The increased vessels wall gradient found in collecting lymphatics was reconciled by the findings that these microlymphatic vessels tend to be contiguous to the arterioles, whereas the terminal lymphatics are dispersed in the tissue. These findings indicate that terminal lymphatic present the lowest oxygen tension in the tissue, and therefore are the locations at risk to develop anoxia when the microvascular oxygen supply becomes limited.