Early experience modifies the postnatal assembly of autonomic emotional motor circuits in rats'
Early experience modifies the postnatal assembly of autonomic emotional motor circuits in rats'
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DOI：
10.1523/jneurosci.2345-05.2005
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发表时间：
2005-10-05
影响因子：
5.3
通讯作者：
Rinaman, L
中科院分区：
文献类型：
--
作者：
Card, JP;Levitt, P;Rinaman, L
反复处理并与母体分离的幼鼠表现出对应激的成年行为、内分泌和自主神经反应的改变，但早期处理和/或母体分离（H/S）在多大程度上改变了这些反应背后的回路的发育尚不清楚。本研究验证了这一假设，即早期H/S改变了出生后组装的突触在preautonomic情绪运动回路。神经回路的发展是通过伪狂犬病病毒（PRV）从胃壁的突触依赖性逆行跨神经元转运来追踪的。对照组和H/S大鼠出生后第6天（P6）和P10，一个时期的快速突触组装前自主电路组件之间进行了分析。从P1开始，每天将H/S组中的幼仔从其母鼠中取出15 min或3 h，并在P8注射病毒，在P10灌注。原发性和跨突触PRV免疫标记的定量分析证实了下丘脑，边缘系统和皮质输入自主神经核的年龄依赖性组装。电路组装显着改变H/S幼鼠，其中较少的中央杏仁核，终纹床核，内脏皮质的神经元被感染相比，年龄匹配的控制。与此相反，H/S并没有改变下丘脑室旁传入胃自主神经元的组装。H/S相关的减少边缘和皮质transneuronal感染是相似的，每天暴露于15分钟或3小时的母亲分离的幼崽。这些发现支持了这样一种观点，即出生后早期的环境事件可以影响神经回路的形成，这些神经回路提供边缘系统和皮质对自主情绪运动输出的控制。
Rat pups that are repeatedly handled and separated from their dam exhibit altered adult behavioral, endocrine, and autonomic responses to stress, but the extent to which early handling and/or maternal separation (H/S) alters the development of circuits that underlie these responses is unknown. The present study tested the hypothesis that early H/S alters the postnatal assembly of synapses within preautonomic emotional motor circuits. Circuit development was traced by synapse-dependent retrograde transneuronal transport of pseudorabies virus (PRV) from the stomach wall. Control and H/S rats were analyzed between postnatal day 6 (P6) and P10, a period of rapid synaptic assembly among preautonomic circuit components. Pups in H/S groups were removed from their dam daily for either 15 min or 3 h beginning on P1, and were injected with virus on P8 and perfused on P10. Quantitative analyses of primary and transsynaptic PRV immunolabeling confirmed an age-dependent assembly of hypothalamic, limbic, and cortical inputs to autonomic nuclei. Circuit assembly was significantly altered in H/S pups, in which fewer neurons in the central amygdala, the bed nucleus of the stria terminalis, and visceral cortices were infected compared with age-matched controls. In contrast, H/S did not alter the assembly of paraventricular hypothalamic inputs to gastric autonomic neurons. H/S-related reductions in limbic and cortical transneuronal infection were similar in pups exposed daily to 15 min or 3 h maternal separation. These findings support the view that environmental events during early postnatal life can influence the formation of neural circuits that provide limbic and cortical control over autonomic emotional motor output.