A TOPOLOGICAL TREATMENT OF RECOMBINATION AND TOPOISOMERASES
A TOPOLOGICAL TREATMENT OF RECOMBINATION AND TOPOISOMERASES
复制标题
DOI：
10.1101/sqb.1984.049.01.045
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
WHITE, JH
中科院分区：
文献类型：
--
作者：
COZZARELLI, NR;KRASNOW, MA;WHITE, JH
目前正在研究的几种位点特异性重组系统的一个特点是，除了重新排列DNA序列外，它们还可以减少底物的超卷曲，并产生结或链状链。通过分析拓扑变化可以揭示重组机制的具体信息。在拓扑异构酶的超卷曲或松弛过程中，环状DNA拓扑结构的变化被很好地定义为DNA连接数（Lk）的变化。没有这样的拓扑结构指标来衡量拓扑异构酶或重组酶在打结或连环化过程中所产生的拓扑变化。
A feature of several site-specific recombination systems currently under study is that they can reduce the supercoiling of the substrate and produce knots or catenanes in addition to rearranging DNA sequences. The topological changes can be analyzed to reveal specific information about the mechanism of recombination.The change in the topology of circular DNA during supercoiling or relaxation by a topoisomerase is welldefined as a change in the linking number (Lk) of the DNA. No such index of topological structure exists to measure the topological changes made during knotting or catenation by either topoisomerases or recombination enzymes.