Behavioral and genetic features of sleep ontogeny in Drosophila
Behavioral and genetic features of sleep ontogeny in Drosophila
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DOI：
10.1093/sleep/zsy086
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发表时间：
2018-07-01
期刊：
影响因子：
5.6
通讯作者：
Kayser, Matthew S.
中科院分区：
文献类型：
--
作者：
Dilley, Leela C.;Vigderman, Abigail;Kayser, Matthew S.
与大多数生物体一样，果蝇幼年时的睡眠时间和深度比成熟动物的睡眠时间和深度都增加。虽然果蝇已成为研究发育过程中睡眠的强大模型，但人们对睡眠个体发育的定性行为特征及其遗传控制知之甚少。在这里，我们发现，除了睡眠时间和强度增加之外，幼龄果蝇的睡眠位置偏好比成熟的成虫更少，并且像哺乳动物一样，在睡眠期间表现出更多的运动抽搐。此外，我们还发现，在不同分子途径存在损伤的睡眠突变体中，睡眠量、抽搐和位置偏好的个体发生变化得以保留。我们的结果表明，睡眠个体发育的特点是多方面的行为变化，包括随着动物成熟睡眠的量和质的改变。此外，尽管突变改变了睡眠时间，但睡眠个体发生变化的保留表明了睡眠成熟的独立遗传控制机制。意义声明我们的数据将果蝇的高分辨率行为跟踪与视频监测相结合，为早期发育过程中的睡眠提供了新的见解，揭示了幼年动物睡眠的保守特征。我们的研究结果支持这样的观点：调节睡眠个体发育的基因与调节整体睡眠持续时间的基因不同。加深我们对生命早期睡眠的理解与研究大脑发育中的睡眠功能密切相关。
The fruit fly Drosophila melanogaster, like most organisms, exhibits increased sleep amount and depth in young compared to mature animals. While the fly has emerged as a powerful model for studying sleep during development, qualitative behavioral features of sleep ontogeny and its genetic control are poorly understood. Here we find that, in addition to increased sleep time and intensity, young flies sleep with less place preference than mature adults, and, like mammals, exhibit more motor twitches during sleep. In addition, we show that ontogenetic changes in sleep amount, twitch, and place preference are preserved across sleep mutants with lesions in distinct molecular pathways. Our results demonstrate that sleep ontogeny is characterized by multifaceted behavioral changes, including quantitative and qualitative alterations to sleep as animals mature. Further, the preservation of sleep ontogenetic changes despite mutations that alter sleep time suggests independent genetic control mechanisms for sleep maturation.Statement of SignificanceOur data combining high-resolution behavioral tracking with video monitoring in Drosophila provide novel insight into sleep during early development, revealing conserved features of sleep in young animals. Our findings support the idea that genes regulating sleep ontogeny are distinct from those that regulate overall sleep duration. Deepening our understanding of early life sleep is highly relevant towards studying the function for sleep in the developing brain.