SPATIAL AND TEMPORAL CONTROLS OF THE ASPERGILLUS BRLA DEVELOPMENTAL REGULATORY GENE
SPATIAL AND TEMPORAL CONTROLS OF THE ASPERGILLUS BRLA DEVELOPMENTAL REGULATORY GENE
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DOI：
10.1111/j.1365-2958.1993.tb01565.x
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发表时间：
1993-04-01
影响因子：
3.6
通讯作者：
AGUIRRE, J
中科院分区：
文献类型：
--
作者：
AGUIRRE, J
构巢曲霉brlA基因编码在控制分生孢子发育中具有重要意义的转录调节因子。我已经确定了其他发育调控基因突变对brlA-lacZ融合基因表达的影响。缺失brlA使β-半乳糖苷酶水平降低一半，并导致酶积累的离域。medA 26和abaA 2发育突变导致融合基因的过表达而不改变空间特异性。与此相反，stuA 1突变并不影响诱导过程中brlA表达的时间或水平，而是导致表达的空间紊乱。这些结果和突变体的表型表明一种模型，其中形态发生基因座的子集由调节基因产物的不同水平和组合控制，这些调节基因产物本身由调节基因之间的相互作用决定。
The Aspergillus nidulans brlA gene encodes a transcriptional regulator of central importance in controlling conidiophore development. I have determined the effects of mutations in other developmental regulatory genes on expression of a brlA-lacZ fusion gene. Deletion of brlA reduced beta-galactosidase levels by half and led to delocalization of enzyme accumulation. The medA26 and abaA2 developmental mutations led to overexpression of the fusion gene without altering spatial specificity. In contrast, the stuA1 mutation did not affect the timing or levels of brlA expression during induction, but instead resulted in spatial derangement of expression. These results and the phenotypes of the mutants suggest a model in which subsets of morphogenetic loci are controlled by differing levels and combinations of regulatory gene products, which are themselves determined by interactions among the regulatory genes.