ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium
ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium
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DOI：
10.1017/s0022149x9900061x
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发表时间：
1999-12-01
影响因子：
1.6
通讯作者：
Margono, SS
中科院分区：
文献类型：
--
作者：
Ito, A;Plancarte, A;Margono, SS
建立可靠的猪囊尾蚴病血清学方法对于监测、控制和预防人和猪的绦虫病/囊尾蚴病以防止经济损失具有重要意义。 ELISA 和免疫印迹均使用通过制备型等电聚焦一步纯化的糖蛋白 (GP)，对人类囊尾蚴病非常有用，已应用于自然感染猪带绦虫的猪的血清学研究。所有猪血清都表现出与人类囊尾蚴病相似的反应。因此，预计 ELISA 和使用 GP 的免疫印迹将有助于区分受感染的猪和未受感染的猪。
The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected with Taenia solium. All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones.