Difference between various copper-containing enzymes (Polyporus laccase, mushroom tyrosinase and cucumber ascorbate oxidase) and bilirubin oxidase.
Difference between various copper-containing enzymes (Polyporus laccase, mushroom tyrosinase and cucumber ascorbate oxidase) and bilirubin oxidase.
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各种含铜酶（猪苓漆酶、蘑菇酪氨酸酶和黄瓜抗坏血酸氧化酶）与胆红素氧化酶的区别。
DOI：
10.1271/bbb1961.47.1627
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
S. Murao
中科院分区：
文献类型：
--
作者：
N. Tanaka;S. Murao
许多酚类物质是抗坏血酸氧化酶的良好底物。3）因此，我们研究了漆酶、酪氨酸酶和抗坏血酸氧化酶是否可以氧化胆红素。4）采用Fahraeus和Reinhammar法从杂色多孔菌（Polyporus versicolor）IFO 9791的培养滤液中分离纯化漆酶，并对纯化的漆酶进行聚丙烯酰胺凝胶圆盘电泳，以确定漆酶的均一性。获得单一蛋白质条带，因此可将其视为特异性的。1 * 酪氨酸酶（Ⅲ级）购自Sigma化学公司，经DEAE-Sephadex A-50、羟基磷灰石和DEAE-Sephadex A-50柱层析纯化。6）7）纯化的酶在聚丙烯酰胺凝胶圆盘电泳上产生单一条带（图1），所以它可以被认为是均匀的。
Many phenolic substances are quite good substrates for ascorbate oxidase.3) Consequently, we examined whether laccase, tyrosinase and ascorbate oxidase can oxidize bilirubin. Brodersen and Bartels have reported that coeruloplasmin cannot oxidize bilirubin.4) Laccase was purified from a culture filtrate of Polyporus versicolor IFO 9791 by the procedure of Fahraeus and Reinhammar.5) The homogeneity of the purified enzyme was examined by means of polyacrylamide gel disc electrophoresis. A single protein band was obtained, so that it could be regarded as homogeneous.1* Tyrosinase (grade III) from mushrooms was purchased from Sigma Chemical Co. and purified by a procedure involving DEAE-Sephadex A-50, hydroxyapatite and DEAE-Sephadex A-50 column chromatographies.6)7) The purified enzyme gave a single band on polyacrylamide gel disc electrophoresis (Fig. 1), so that it could be regarded as homogeneous.