SUBUNIT COMMUNICATION IN THE ANTHRANILATE SYNTHASE COMPLEX FROM SALMONELLA-TYPHIMURIUM
SUBUNIT COMMUNICATION IN THE ANTHRANILATE SYNTHASE COMPLEX FROM SALMONELLA-TYPHIMURIUM
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DOI：
10.1126/science.2063197
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发表时间：
1991-06-28
期刊：
影响因子：
56.9
通讯作者：
BAUERLE, R
中科院分区：
文献类型：
--
作者：
CALIGIURI, MG;BAUERLE, R
鼠伤寒沙门氏菌中色氨酸生物合成途径的邻氨基苯甲酸合酶-磷酸核糖基转移酶复合物是一种变构异源四聚体（TrpE 2-TrpD 2）酶，其多种活性受L-色氨酸负反馈调节。在体外组装了一种含有一个催化活性，反馈不敏感和一个催化失活，反馈敏感的突变型TrpE亚基的混合复合物，并用于研究位于不同亚基上的调节和催化位点之间的通信。的混合复合物的性质表明，一个单一的抑制剂分子的一个TrpE亚基的结合是足以传播的构象变化，影响伴侣亚基的活性位点。
The anthranilate synthase - phosphoribosyl transferase complex of the tryptophan biosynthetic pathway in Salmonella typhimurium is an allosteric, heterotetrameric (TrpE2-TrpD2) enzyme whose multiple activities are negatively feedback-regulated by L-tryptophan. A hybrid complex containing one catalytically active, feedback-insensitive and one catalytically inactive, feedback-sensitive mutant TrpE subunit was assembled in vitro and used to investigate communication between regulatory and catalytic sites located on different subunits. The properties of the hybrid complex demonstrate that the binding of a single inhibitor molecule to one TrpE subunit is sufficient for the propagation of a conformational change that affects the active site of the companion subunit.