The biological clock of an hematophagous insect: Locomotor activity rhythms, circadian expression and downregulation after a blood meal
The biological clock of an hematophagous insect: Locomotor activity rhythms, circadian expression and downregulation after a blood meal
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DOI：
10.1016/j.febslet.2005.11.031
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发表时间：
2006-01-09
期刊：
影响因子：
3.5
通讯作者：
Peixoto, AA
中科院分区：
文献类型：
--
作者：
Meireles, ACA;Rivas, GBD;Peixoto, AA
尽管昼夜节律在媒介疾病传播中很重要，但人们对其对食血昆虫媒介的分子控制知之甚少。在果蝇中，基因表达的负反馈回路已被证明有助于时钟机制。在这里，我们描述了白蛉（内脏利什曼病的媒介）的生物钟的一些特征。与黑腹果蝇相比，白蛉时期和永恒这两个反馈环路的负面元素显示出相似的 mRNA 丰度峰值。另一方面，时钟（正转录因子）的表达在两个物种之间存在差异，这提高了时钟表达的不同阶段可能与观察到的昼夜活动节律差异相关的可能性。此外，我们发现吸血后运动活动减少，这与 period 和 timeless 表达水平的下调相关。我们的结果表明，这种食血昆虫的昼夜节律起搏器及其对活动节律的控制受到血液摄入量的调节。 (c) 2005 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Despite the importance of circadian rhythms in vector-home disease transmission, very little is known about its molecular control in hematophagous insect vectors. In Drosophila melanogaster, a negative feedback loop of gene expression has been shown to contribute to the clock mechanism. Here, we describe some features of the circadian clock of the sandfly Lutzomyia longipalpis, a vector of visceral leishmaniasis. Compared to D. melanogaster, sandfly period and timeless, two negative elements of the feedback loop, show similar peaks of mRNA abundance. On the other hand, the expression of Clock (a positive transcription factor) differs between the two species, raising the possibility that the different phases of Clock expression could be associated with the observed differences in circathan activity rhythms. In addition, we show a reduction in locomotor activity after a blood meal, which is correlated with downregulation of period and timeless expression levels. Our results suggest that the circadian pacemaker and its control over the activity rhythms in this hematophagous insect are modulated by blood intake. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.