Cytokine-induced killer cells as a feasible adoptive immunotherapy for the treatment of lung cancer.
Cytokine-induced killer cells as a feasible adoptive immunotherapy for the treatment of lung cancer.
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细胞因子诱导的杀伤细胞作为治疗肺癌的可行过继免疫疗法
DOI：
10.1038/s41419-018-0404-5
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发表时间：
2018-03-06
影响因子：
9
通讯作者：
Feng J
中科院分区：
文献类型：
--
作者：
Chen D;Sha H;Hu T;Dong S;Zhang J;Liu S;Cao H;Ma R;Wu Y;Jing C;Wang Z;Wu J;Feng J
肺癌患者多为晚期诊断，往往失去手术治疗的机会，多数患者化疗后预后不佳。近年来，一些临床研究证实了过继性t细胞转移在癌症患者维持治疗中的作用。在这里，我们从细胞模型（体外共培养系统）、小鼠模型（原位肺癌）和临床研究（不同进展阶段的肺癌患者）三个不同的水平提供了CIKs在晚期肺癌中的作用的统计见解。我们优化了补充剂成分和细胞因子对CIK细胞激活和扩增的作用。在此基础上，我们探索了一种新的体外激活和扩增CIK细胞的无血清培养基。此外，我们在体外细胞-细胞模型中发现，激活的CIK细胞可以有效地杀死肺癌细胞，并显著降低小鼠肿瘤的生长。在临床研究中，化疗联合CIK治疗患者的OS率明显高于单纯化疗患者的OS率。此外，在我们的研究中，CIK治疗表现出良好的耐受性。这些结果表明，免疫治疗与传统治疗相结合将是一种可行的、有前景的治疗肺癌的方法。
Most of the patients with lung cancer are diagnosed at advanced stage, and they often lose the opportunity of surgical therapy, most of whom fail to reach good prognosis after chemotherapy. Recently, a few clinical studies have confirmed the role of adoptive T-cell transfer in the maintenance therapy of cancer patients. Here, we provided statistical insights into the role of CIKs in advanced lung cancer from three different levels, cell model (in vitro co-culture system), mice model (in situ lung cancer), and clinical research (in lung cancer patients of different progression stages). We optimized the components of supplements and cytokines on activating and expanding CIK cells. Based on this, we explored a new serum-free medium for in vitro activation and expansion of CIK cells. Moreover, we found that activated CIK cells could efficiently kill lung cancer cells in cell-to-cell model in vitro and significantly reduce the tumor growth in mice. For the clinical research, the OS rates of patients received combination of chemotherapy and CIK treatment were significantly improved compared to the OS rates of patients only received chemotherapy. Additionally, CIK therapy represented good toleration in our study. All the results suggested that combination of immunotherapy with traditional therapy will be a feasible and promising method for the treatment of lung cancer.
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影响因子：
3.6
作者：
Lee, Dong Hyeon;Nam, Joon Yeul;Yoon, Jung-Hwan
通讯作者：
Yoon, Jung-Hwan
DOI：
10.1158/1078-0432.ccr-17-1185
发表时间：
2017-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Chen Y;Xue SA;Behboudi S;Mohammad GH;Pereira SP;Morris EC
通讯作者：
Morris EC
影响因子：
7.5
作者：
Luo, Hu;Gong, Liang;Zhou, Xiangdong
通讯作者：
Zhou, Xiangdong
影响因子：
2.4
作者：
Du, Lingling;Herbst, Roy S.;Morgensztern, Daniel
通讯作者：
Morgensztern, Daniel
DOI：
10.1186/1479-0556-2-12
发表时间：
2004
期刊：
Genetic vaccines and therapy
影响因子：
--
作者：
Nagaraj S;Ziske C;Schmidt-Wolf IG
通讯作者：
Schmidt-Wolf IG