HYALURONAN AFFECTS EXTRAVASCULAR WATER IN LUNGS OF UNANESTHETIZED RABBITS
HYALURONAN AFFECTS EXTRAVASCULAR WATER IN LUNGS OF UNANESTHETIZED RABBITS
复制标题
DOI：
10.1152/jappl.1989.66.6.2595
复制
发表时间：
1989-06-01
影响因子：
3.3
通讯作者：
BRAY, BA
中科院分区：
文献类型：
--
作者：
BHATTACHARYA, J;CRUZ, T;BRAY, BA
我们已经确定了肺透明质酸含量的变化是否会影响未麻醉家兔肺血管外水。进行了三组实验。在第1组（n = 12）中，不给予输注;在第2组中，9对兔接受静脉内透明质酸酶（750 U. kg-1. min-1）或等体积的生理盐水：在第3组中，9对家兔接受透明质酸酶或生理盐水，然后静脉输注相当于体重24%的生理盐水。在每个实验结束时，通过湿-干方法分析一个肺的血管外肺水。除第3组外，在所有动物中，通过涉及用真菌透明质酸酶水解肺透明质酸以释放定量的还原性N-乙酰葡糖胺基团的方法分析另一个肺的透明质酸含量。在第1组中，肺透明质酸，从50 μ g/g干重变化到159 μ g/g干重（平均106 ± 100 μ g/g干重）。35 μ g/g干重），与血管外肺水的变化显著相关（平均值4.2 ± 0.05 μ g/g干重）。0.3 g/g干重）。实验2组兔肺组织透明质酸含量和血管外肺含水量分别比对照组低40%和14.6%（P < 0.01）。在第3组中，容量扩张不影响肺水，除了在透明质酸酶后肺水比配对对照高47%。我们的结论是，在肺内容的透明质酸是血管外水含量的决定因素之一。
We have determined whether changes in lung hyaluronan content affect extravascular water in lungs of unanesthetized rabbits. Three groups of experiments were performed. In group 1 (n = 12), no infusions were given; in group 2, nine pairs of rabbits received either intravenous hyaluronidase (750 U .cntdot. kg-1 .cntdot. min-1) or an equivalent volume of saline: in group 3, nine pairs of rabbits received either hyaluronidase or saline, followed by intravenous saline infusion amounting to 24% of body weight. At the end of each experiment, one lung was analzyed for extravascular lung water by the wet-dry method. Except for group 3, in all animals the other lung was analyzed for hyaluronan content by a method that involved hydrolyzing lung hyaluronan with fungal hyaluronidase to release reducing N-acetyl glucosamine groups, which were quantified. In group 1, lung hyaluronan, which varied from 50 to 159 .mu.g/g dry wt (mean 106 .+-. 35 .mu.g/g dry wt), significantly correlated with variation in extravascular lung water (mean 4.2 .+-. 0.3 g/g dry wt). In group 2 rabbits given hyaluronidase, lung hyaluronan was 40% lower and extravascular lung water was 14.6% lower than in paired controls (P < 0.01). In group 3, volume expanison did not affect lung water, except after hyaluronidase when lung water was 47% higher than paired controls. We conclude that in the lung content of hyaluronan is one of the determinants of extravascular water content.