A cortical rat hemodynamic response function for improved detection of BOLD activation under common experimental conditions
A cortical rat hemodynamic response function for improved detection of BOLD activation under common experimental conditions
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DOI：
10.1016/j.neuroimage.2019.116446
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发表时间：
2020-03-01
期刊：
影响因子：
5.7
通讯作者：
Faber, Cornelius
中科院分区：
文献类型：
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作者：
Lambers, Henriette;Segeroth, Martin;Faber, Cornelius
对于基于BOLD fMRI测量的神经元激活的可靠估计，精确的血流动力学反应模型是必不可少的。由于神经科学基础研究的很大一部分是基于小动物数据，因此有必要表征针对小动物优化的血流动力学反应函数（HRF）。因此，我们测定并研究了在各种实验条件下获得的大鼠初级体感皮层的hrf。对不同性别和品系的动物，在不同的麻醉药下，使用和不使用通气以及使用不同的刺激方式进行了测量。本研究中使用的所有刺激方式都能诱导初级体感觉皮层或皮层下区域的神经元活动。由于初级体感觉皮层的BOLD反应的HRF在不同条件下显示出密切的一致性，我们能够确定皮质大鼠的HRF。该HRF基于76只大鼠的143个BOLD测量值，可用于统计参数映射。它的HRF进展明显快于人类，在2.8 +/- 0.8 s后达到最大值，在6.1 +/- 3.7 s后达到最低点。如果使用大鼠HRF，对大鼠数据的统计分析显示，与基于人类测量的常用HRF相比，躯体感觉皮层的检测性能显着提高。
For a reliable estimation of neuronal activation based on BOLD fMRI measurements an accurate model of the hemodynamic response is essential. Since a large part of basic neuroscience research is based on small animal data, it is necessary to characterize a hemodynamic response function (HRF) which is optimized for small animals. Therefore, we have determined and investigated the HRFs of rats obtained under a variety of experimental conditions in the primary somatosensory cortex. Measurements were performed on animals of different sex and strain, under different anesthetics, with and without ventilation and using different stimulation modalities. All modalities of stimulation used in this study induced neuronal activity in the primary somatosensory cortex or in subcortical regions. Since the HRFs of the BOLD responses in the primary somatosensory cortex showed a close concordance for the different conditions, we were able to determine a cortical rat HRF. This HRF is based on 143 BOLD measurements of 76 rats and can be used for statistical parametric mapping. It showed substantially faster progression than the human HRF, with a maximum after 2.8 +/- 0.8 s, and a following undershoot after 6.1 +/- 3.7 s. If the rat HRF was used statistical analysis of rat data showed a significantly improved detection performance in the somatosensory cortex in comparison to the commonly used HRF based on measurements in humans.