PROTECTION OF COTTONTOP TAMARINS AGAINST EPSTEIN-BARR VIRUS-INDUCED MALIGNANT-LYMPHOMA BY A PROTOTYPE SUBUNIT VACCINE
PROTECTION OF COTTONTOP TAMARINS AGAINST EPSTEIN-BARR VIRUS-INDUCED MALIGNANT-LYMPHOMA BY A PROTOTYPE SUBUNIT VACCINE
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DOI：
10.1038/318287a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
KIRKWOOD, JK
中科院分区：
文献类型：
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作者：
EPSTEIN, MA;MORGAN, AJ;KIRKWOOD, JK
EB病毒（Epstein-Barr virus，EB）是人类五种疱疹病毒之一，它与引起地方性伯基特淋巴瘤和未分化鼻咽癌两种人类癌症的一系列事件之间的密切联系早已得到证实。由于这一点，以及由于鼻咽癌在某些大人口中的发病率非常高2，1976年有人建议应开发针对EB病毒的疫苗，以预防感染，从而降低高危人群中的肿瘤发病率3。病毒决定的膜抗原（MA）被提议作为免疫原3，因为已知它可以在人体中引发天然存在的病毒中和抗体4 - 7，并且因为类似的抗原已被证明可以作为有效的实验疫苗，用于预防鸡马立克氏病的疱疹病毒诱导的淋巴瘤8，9。在MA分子的定义、定量和制备以及增强其免疫原性方面取得了进展10;已详细阐述了MA抗体的灵敏测定11。在这里，我们报告说，分离的细胞膜表达MA，或纯化的MA糖蛋白的相对分子质量（Mr）340，000（gp 340），已被用于接种棉顶绢毛猴（Saguinus oedipus oedipus），动物接受任何一种制剂的保护，免受100%的肿瘤诱导的EB病毒的攻击剂量的影响。
Epstein–Barr (EB) virus is one of the five herpesviruses of man. Strong links between this agent and the chain of events causing two human cancers, endemic Burkitt's lymphoma and undifferentiated nasopharyngeal carcinoma, have long been evident (reviewed in ref. 1). Because of this, and because of the very high incidence of nasopharyngeal carcinoma in certain large populations2, it was suggested in 1976 that a vaccine should be developed against EB virus to prevent infection and thereby reduce tumour incidence amongst those at risk3. The virus-determined membrane antigen (MA) was proposed as immunogen3because it was known to elicit naturally occurring virus-neutralizing antibodies in man4–7and because analogous antigens had been shown to act as effective experimental vaccines for preventing the herpesvirus-induced lymphomas of Marek's disease in chickens8,9. Progress has been achieved in defining, quantifying and preparing MA molecules, and in enhancing their immunogenicity10; a sensitive assay for antibodies to MA has been elaborated11. Here we report that isolated cell membranes expressing MA, or purified MA glycoprotein of relative molecular mass (Mr) 340,000 (gp340), have been used to vaccinate cottontop tamarins (Saguinus oedipus oedipus), and that animals receiving either preparation were protected against the effects of a 100% tumour-inducing challenge dose of EB virus.