In vitro proliferation and production of gamma interferon by murine CD4+ cells in response to Cryptosporidium parvum antigen.
In vitro proliferation and production of gamma interferon by murine CD4+ cells in response to Cryptosporidium parvum antigen.
复制标题
小鼠 CD4 细胞响应小隐孢子虫抗原而体外增殖和产生 γ 干扰素。
作者：
J. Harp;W. Whitmire;R. Sacco
通过在亲和层析柱上传代来富集来自用微小隐孢子虫免疫的小鼠的脾细胞的T细胞。这些细胞的增殖反应比未富集细胞的反应高> 2倍。T富集细胞进一步富集CD 4+细胞或CD 8+细胞。CD 4富集细胞的增殖反应比未富集细胞的反应高> 4倍。CD 8+细胞对C.细小病毒抗原测定来自这些不同富集的脾细胞群的培养物上清液级分的细胞因子产生。含有CD 4+细胞的培养物在与C.细小病毒抗原没有一种培养物产生白细胞介素-4。产生γ干扰素和白细胞介素-2，但不产生白细胞介素-4，是先前描述的Th 1辅助细胞亚群的特征。我们的数据表明，一个子集的小鼠淋巴细胞与Th 1辅助细胞表型一致增殖后，在体外刺激与C。细小病毒抗原
Spleen cells from mice immunized with Cryptosporidium parvum were enriched for T cells by passage over an affinity chromatography column. The proliferative response of these cells was > 2-fold higher than the response of unenriched cells. T-enriched cells were enriched further for either CD4+ cells or CD8+ cells. The proliferative response of CD4-enriched cells was > 4-fold higher than the response by unenriched cells. CD8+ cells were essentially nonresponsive to C. parvum antigen. Culture supernatant fractions from these variously enriched splenocyte populations were assayed for cytokine production. Cultures containing CD4+ cells produced gamma interferon and interleukin-2 following incubation with C. parvum antigen. None of the cultures produced interleukin-4. Production of gamma interferon and interleukin-2, but not interleukin-4, is characteristic of the previously described Th1 helper cell subset. Our data indicate that a subset of murine lymphocytes consistent with the Th1 helper cell phenotype proliferates following in vitro stimulation with C. parvum antigen.
IFN-gamma 调节皮肤利什曼病小鼠模型中 Th1 和 Th2 反应的早期发展。
DOI：
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发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Scott,P
通讯作者：
Scott,P