In vitro differentiation of rabbit blastocyst cells.
In vitro differentiation of rabbit blastocyst cells.
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兔囊胚细胞的体外分化。
DOI：
10.1007/bf02624628
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Benos,DJ
中科院分区：
文献类型：
--
作者：
Gargus,EB;Robinson,DH;Bubien,JK;Bugaisky,LB;Benos,DJ
交配后6天和7天（p.c.）培养兔胚胎以试图建立滋养外胚层细胞系。结果表明，具有上皮特征（即细胞角蛋白染色阳性）的细胞将在培养物中存活至第3代。此时成纤维细胞样细胞占优势。此外，我们发现胚胎外植体需要内细胞团的存在，这通常会导致自发收缩的细胞的发育。这些细胞对肌球蛋白呈阳性染色，这表明它们可能是心前细胞。最大舒张电位为−59±1.2 mV，阈值电位为−53±2.3 mV。自发收缩细胞对阿托品、乙酰胆碱、肾上腺素、异丙肾上腺素或普萘洛尔无反应。动作电位似乎是内向钙电流的结果，因为钙通道阻滞剂维拉帕米以剂量相关的方式降低了搏动率，而电压敏感性钠通道阻滞剂河豚毒素则没有影响。
Six- and seven-day post-coitus (p.c.) rabbit embryos have been cultured in an attempt to establish a trophectodermal cell line. Results indicate that cells with epithelial characteristics (i.e. positive staining for cytokeratin) will survive in culture until Passage 3. At that time a fibroblastlike cell becomes predominant. In addition, we have found that the presence of the inner cell mass is required for embryo explants often results in the development of cells that spontaneously contract. These cells stain positively for myosin, which indicates that they may be precardiac cells. Maximum diastolic potential was −59±1.2 mV and the threshold potential was −53±2.3 mV. Spontaneously contracting cells did not respond to atropine, acetylcholine, epinephrine, isoproterenol, or propranolol. Action potential seems to be a result of an inward calcium current, because the beating rate is decreased in a dose-related manner with the calcium channel blocker verapamil, whereas the voltage-sensitive sodium channel blocker tetrodotoxin was without effect.