The P3 Protein of Turnip mosaic virus Can Alone Induce Hypersensitive Response-Like Cell Death in Arabidopsis thaliana Carrying TuNI
The P3 Protein of Turnip mosaic virus Can Alone Induce Hypersensitive Response-Like Cell Death in Arabidopsis thaliana Carrying TuNI
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DOI：
10.1094/mpmi-23-2-0144
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发表时间：
2010-02-01
影响因子：
3.5
通讯作者：
Masuta, Chikara
中科院分区：
文献类型：
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作者：
Kim, Bo Min;Suehiro, Noriko;Masuta, Chikara
芜菁花叶病毒（Turnip mosaic virus，TuMV）TuR 1和TuC株系在拟南芥（Arabidopsis thaliana）生态型兰茨贝格直立（Landsberg erecta，Ler）上诱导不同的症状，TuR 1株系引起植株全身坏死，TuC株系引起花叶。我们以前发现，Ler系统性坏死是由一个单一的显性基因，TuNI（TuMV坏死诱导剂）控制，它实际上是一种形式的宿主防御反应，导致过敏反应（HR）样细胞死亡。为了鉴定与TuNI相互作用的病毒因子，进行了TuR1和TuC基因组克隆之间的结构域交换，并且我们鉴定了与TuNI相互作用的TuMV症状决定簇为P3基因。此外，我们还发现P3基因的0.5kb结构域，包括3个不同的氨基酸，是导致系统性坏死的原因。为了验证P3基因可以单独诱导坏死，我们分析了P3基因在Ler转基因植株中的组成型表达和在Ler原生质体中的瞬时表达。这些结果表明，P3单独引起HR样细胞死亡。在这项研究中，我们成功地证明了系统性坏死TuMV在拟南芥是由TuNI和P3之间的基因对基因的相互作用，使用原生质体系统进行直接验证。
Strains TuR1 and TuC of Turnip mosaic virus (TuMV) induce different symptoms on Arabidopsis thaliana ecotype Landsberg erecta (Ler); plants infected with TuR1 develop systemic necrosis, while TuC causes mosaics. We previously found that the Ler systemic necrosis was controlled by a single dominant gene, TuNI (TuMV necrosis inducer), and that it was actually a form of host defense response leading to a hypersensitive reaction (HR)-like cell death. To identify the viral factor interacting with TuNI, the domain swapping between the genomic clones of TuR1 and TuC was carried out, and we identified the TuMV symptom determinant interacting with TuNI as the P3 gene. Moreover, it was found that the central 0.5-kb domain of P3, including three different amino acids between the two isolates, was responsible for the systemic HR. To verify that the P3 gene can alone induce necrosis, we analyzed the constitutive P3 expression in Ler transgenic plants and the transient P3 expression in Ler protoplasts. These results indicated that P3 alone caused HR-like cell death. In this study, we successfully demonstrated that the systemic necrosis by TuMV in Arabidopsis was determined by the gene-for-gene interaction between TuNI and P3 using the protoplast system for direct verification.