Structural and SAXS analysis of the budding yeast SHU-complex proteins
Structural and SAXS analysis of the budding yeast SHU-complex proteins
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芽殖酵母 SHU 复合蛋白的结构和 SAXS 分析
DOI：
10.1016/j.febslet.2012.06.024
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发表时间：
2012-07-30
期刊：
影响因子：
3.5
通讯作者：
Dong, Yu-Hui
中科院分区：
文献类型：
--
作者：
She, Zhun;Gao, Zeng-Qiang;Dong, Yu-Hui
在酿酒酵母中，Shu1、Shu2、Squ3和Csm2四种蛋白质在体内和体外都形成稳定的SHU-复合体。这些蛋白质参与同源重组DNA损伤修复过程的早期阶段。在本文中，我们在1.8埃分辨率下给出了该化合物的晶体结构，并成功地将其与我们的小角X射线散射(SAXS)数据进行了拟合。结合我们的凝胶迁移率改变分析(EMSA)结果，提出了SHU-蛋白质与DNA偶联的模型。
In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8 angstrom resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA.