Signature lipids and stable carbon isotope analyses of octopus spring hyperthermophilic communities compared with those of Aquificales representatives
Signature lipids and stable carbon isotope analyses of octopus spring hyperthermophilic communities compared with those of Aquificales representatives
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章鱼泉超嗜热生物群落的特征脂质和稳定碳同位素分析与 Aquificales 代表生物群落的特征脂质和稳定碳同位素分析的比较
DOI：
10.1128/aem.67.11.5179-5189.2001
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发表时间：
2001-11-01
影响因子：
4.4
通讯作者：
Summons, RE
中科院分区：
文献类型：
--
作者：
Jahnke, LL;Eder, W;Summons, RE
利用水生目（Aquificales）属生物标志物的分子和同位素组成研究了章鱼泉（Octopus Spring）超嗜热粉红飘带和喷口生物膜的群落和营养结构。红色嗜热菌、嗜热菌属菌株HI 11/12、嗜热嗜氢菌TK-6、嗜热产液菌和风产液菌均含有甘油-醚磷脂以及酰基甘油酯。n-C-20：1和cy-C-21脂肪酸占优势，而烷基甘油醚主要是C-18：0。这些Aquificales生物标志物是两个Octopus Spring样品的脂质提取物的主要成分，与硅质喷口壁相关的生物膜，以及著名的粉红色流光群落（PSC）。生物膜和PSC都含有单烷基和二烷基甘油醚，其中C-18和C-20烷基是普遍存在的。磷脂脂肪酸包括Aquificales n-C-20：1和cy-C-21，加上一系列同支脂肪酸（i-C-15：0至i-C-21：0），表明另外的细菌组分。生物质和脂质从PSC耗尽C-13相对于源水CO2的10.9和17.2千分之一，分别。PSC的C20-21脂肪酸比异支链脂肪酸消耗较少，分别为18.4和22.6份/千。T.相对于C源，在CO2上生长的橡胶中C-13的消耗量仅为千分之3.3。相比之下，当甲酸盐是C源时，生物质被消耗了19.7份。不依赖碳源，T.红脂比生物量重（+1.3ppm）。PSC中C20-21脂肪酸的消耗表明，Thermocrinis生物质必须类似地被消耗，并且太轻而不能用CO2上的生长来解释。因此，PSC中的Thermocrinis很可能利用了甲酸盐，推测是在泉水源区产生的。
The molecular and isotopic compositions of lipid biomarkers of cultured Aquificales genera have been used to study the community and trophic structure of the hyperthermophilic pink streamers and vent biofilm from Octopus Spring. Thermocrinis ruber, Thermocrinis sp. strain HI 11/12, Hydrogenobacter thermophilas TK-6, Aquifex pyrophilus, and Aquifex aeolicus all contained glycerol-ether phospholipids as well as acyl glycerides. The n-C-20:1 and cy-C-21 fatty acids dominated all of the Aquificales, while the alkyl glycerol ethers were mainly C-18:0, These Aquificales biomarkers were major constituents of the lipid extracts of two Octopus Spring samples, a biofilm associated with the siliceous vent walls, and the well-known pink streamer community (PSC). Both the biofilm and the PSC contained mono- and dialkyl glycerol ethers in which C-18 and C-20 alkyl groups were prevalent. Phospholipid fatty acids included both the Aquificales n-C-20:1 and cy-C-21, plus a series of isobranched fatty acids (i-C-15:0 to i-C-21:0), indicating an additional bacterial component. Biomass and lipids from the PSC were depleted in C-13 relative to source water CO2 by 10.9 and 17.2 parts per thousand, respectively. The C20-21 fatty acids of the PSC were less depleted than the iso-branched fatty acids, 18.4 and 22.6 parts per thousand, respectively. The biomass of T. ruber grown on CO2 was depleted in C-13 by only 3.3 parts per thousand relative to C source. In contrast, biomass was depleted by 19.7 parts per thousand when formate was the C source. Independent of carbon source, T. ruber lipids were heavier than biomass (+1.3 parts per thousand). The depletion in the C20-21 fatty acids from the PSC indicates that Thermocrinis biomass must be similarly depleted and too light to be explained by growth on CO2. Accordingly, Thermocrinis in the PSC is likely to have utilized formate, presumably generated in the spring source region.