A comparative immunocytochemical study of development and regeneration of chemosensory neurons in the rat vomeronasal system.
A comparative immunocytochemical study of development and regeneration of chemosensory neurons in the rat vomeronasal system.
复制标题
大鼠犁鼻系统化学感应神经元发育和再生的比较免疫细胞化学研究。
DOI：
10.1016/s0006-8993(02)02823-8
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发表时间：
2002
期刊：
影响因子：
2.9
通讯作者：
Costanzo,RichardM
中科院分区：
文献类型：
--
作者：
Matsuoka,Masato;Osada,Toshiya;Yoshida-Matsuoka,Junko;Ikai,Atsushi;Ichikawa,Masumi;Norita,Masao;Costanzo,RichardM
犁鼻神经元在发育过程中和神经元损伤后经历连续的神经发生。我们用免疫细胞化学方法比较了犁鼻神经切断后不同阶段的犁鼻器官发育与再生。在E15和损伤后6 ~ 10天，巢蛋白阳性细胞遍布感觉上皮。我们没有发现巢蛋白的免疫反应性局限于上皮的边界区域。巢蛋白阳性细胞的早期出现和广泛分布表明它们代表化学感觉前体细胞，在上皮中垂直发育和迁移。Vomeronasal受体细胞在神经横断后6至8天退化，但副嗅球（AOB）的轴突终末继续显示化学感觉特异性标记（OMP）的存在长达10天，这是本研究中观察到的一个重要发现。与神经横断部位的距离可能导致轴突顺行和逆行退化的时间差异。在正常成人上皮中观察到omp阳性神经元，在神经横断恢复后10-60天观察到omp阳性神经元的程度要小得多。再生受体细胞的轴突在这段时间内没有到达AOB。这种与AOB中靶细胞重建连接的失败可以解释为什么在粪鼻上皮再生细胞中很少观察到omp阳性细胞。
Vomeronasal neurons undergo continuous neurogenesis during development and after neuronal injury. We used immunocytochemical methods to compare different stages of the vomeronasal organ development to those of regeneration following vomeronasal nerve transection. At E15 and at 6 to 10 days after injury, nestin-positive cells were observed throughout the sensory epithelium. We did not find nestin immunoreactivity to be localized to the boundary region of the epithelium. The early appearance and wide distribution of nestin-positive cells suggests that they represent chemosensory precursor cells that develop and migrate vertically in the epithelium. Vomeronasal receptor cells degenerated 6 to 8 days after nerve transection, but axon terminals in the accessory olfactory bulb (AOB) continued to show the presence of the chemosensory specific marker (OMP) for up to ten days, a significant finding observed in this study. It is likely that the distance from the site of nerve transection may contribute to differences in the time course of anterograde and retrograde axon degradation. OMP-positive neurons were observed in the normal adult epithelium and to a much lesser extent 10–60 days after recovery from nerve transection. Axons from regenerated receptor cells did not reach the AOB during this time period. This failure to reestablish connections with target cells in the AOB could explain why OMP-positive cells were rarely observed among the regenerated cells in the vomeronasal epithelium.