Mesangial cell necrosis in Thy 1 glomerulonephritis – an ultrastructural study
Mesangial cell necrosis in Thy 1 glomerulonephritis – an ultrastructural study
复制标题
Thy 1 肾小球肾炎中的系膜细胞坏死——一项超微结构研究
作者：
K. Mosley;J. Collar;V. Cattell
摘要：细胞死亡是许多生理和病理过程的核心。由于组织对两种形式的细胞死亡（坏死和凋亡）的反应不同，因此区分它们至关重要。尽管超微结构仍然是评估这一点的明确手段，但体内研究很少。在大鼠中施用抗Thy 1抗体是由于其表达Thy 1.1表位而导致急性肾小球系膜细胞死亡的模型。这个过程的性质尚不清楚；早期形态学研究和最近抗Thy 1.1抗体的体外作用表明细胞凋亡。我们通过电子显微镜重新检查了这些变化，并确定了在施用抗 Thy1.1 抗体后 30 分钟内开始的细胞坏死过程。虽然存在染色质浓缩，但与细胞凋亡不同的坏死特征是：核膜丢失、细胞肿胀和细胞质细胞器变性，染色质和细胞器释放到间质中，引起急性炎症，而没有吞噬细胞凋亡小体。这些发现符合该模型已知的补体依赖性。超微结构是区分体内坏死和细胞凋亡的重要手段，这对于理解损伤的发病机制和随后的组织反应非常重要。
Abstract Cell death is central to many physiological and pathological processes. As tissue reactions to the two forms of cell death, necrosis and apoptosis, differ, it is critical to distinguish between them. Although ultrastructure is still the definitive means of assessing this, there are very few in vivo studies. Administration of anti-Thy 1 antibody in rats is a model of acute glomerular mesangial cell death due to their expression of the Thy 1.1 epitope. The nature of this process is unclear; apoptosis was suggested from early morphological studies and recent in vitro effects of anti-Thy 1.1 antibody. We have re-examined the changes by electron microscopy, and identified a process of cell necrosis starting within 30 min of anti-Thy1.1 antibody administration. Although there was chromatin condensation, the necrotic features distinctive from apoptosis were: loss of nuclear membranes, cell swelling and degeneration of cytoplasmic organelles, with liberation of chromatin and organelles into the interstitium causing acute inflammation without phagocytic uptake of apoptotic bodies. These findings accord with the known complement dependence of this model. Ultrastructure is a valuable means of differentiating between in vivo necrosis and apoptosis and this is important for understanding the pathogenesis of injury and subsequent tissue responses.
影响因子：
4.4
作者：
T. Yamamoto;C. Wilson
通讯作者：
T. Yamamoto;C. Wilson
影响因子：
19.6
作者：
Brandt, J;Pippin, J;Couser, WG
通讯作者：
Couser, WG
影响因子：
15.9
作者：
BAKER, AJ;MOONEY, A;SAVILL, J
通讯作者：
SAVILL, J