Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based Recognition
Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based Recognition
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DOI：
10.1021/acsami.6b06671
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发表时间：
2016-08-03
影响因子：
9.5
通讯作者：
Yuan, Quan
中科院分区：
文献类型：
--
作者：
Shen, Haijing;Wang, Jie;Yuan, Quan
Sepsis and bacteremia are life-threatening clinical syndromes associated with significant patient morbidity and mortality. Rapid and sensitive detection of pathogenic bacteria is the key to improve patient survival rates.在此，我们合理构建了一个简单的基于适配体的捕获平台，以缩短临床血液样本中细菌血流感染确认所需的时间。 This capture platform is made of a mesoporons TiO2-coated magnetic nanoparticle and is modified with target aptamer. It features excellent bacterial enrichment efficiency of about 80% even at low bacterial concentrations (10-2000 CFU mL(-1)).更重要的是，细菌可在2小时内富集，与临床血流感染诊断的“金标准”相比，有效缩短了细菌鉴定的时间。 The aptamer-based capture platform may pave a way for the detection of biomarkers and find potential applications in disease diagnosis.
Sepsis and bacteremia are life-threatening clinical syndromes associated with significant patient morbidity and mortality. Rapid and sensitive detection of pathogenic bacteria is the key to improve patient survival rates. Herein, we have rationally constructed a simple aptamer-based capture platform to shorten the time needed for confirmation of bacterial bloodstream infection in clinical blood samples. This capture platform is made of a mesoporons TiO2-coated magnetic nanoparticle and is modified with target aptamer. It features excellent bacterial enrichment efficiency of about 80% even at low bacterial concentrations (10-2000 CFU mL(-1)). More importantly, the bacteria can be enriched within 2 h, and the time for bacterial identification is effectively shortened in comparison to the "gold standard" in clinical diagnosis of bloodstream infection. The aptamer-based capture platform may pave a way for the detection of biomarkers and find potential applications in disease diagnosis.