APPARENT [H-3] 1,25-DIHYDROXYVITAMIN-D3 UPTAKE BY CANINE AND RODENT BRAIN
APPARENT [H-3] 1,25-DIHYDROXYVITAMIN-D3 UPTAKE BY CANINE AND RODENT BRAIN
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DOI：
10.1152/ajpendo.1983.244.3.e266
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发表时间：
1983-01-01
影响因子：
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通讯作者：
HUET, PM
中科院分区：
文献类型：
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作者：
GASCONBARRE, M;HUET, PM
采用多指标稀释技术研究了犬在稳态条件下对[3H]1,25-二羟基维生素D3 （[3H]1,25(OH)2D3）的脑摄取。分数[3H]1,25(OH)2D3摄取评估为0.8 .+-。在狗的大脑中单次通过的0.15%。对维生素D充满和缺乏大鼠脑对[3H]1,25(OH)2D3的摄取评价表明，注射后30 min，部分摄取不受动物维生素D状态的影响，也不受[3H]-1,25(OH)2D3脑蓄积量的影响，在0.16-0.20%之间；脑-血清比率在5-6%之间变化。血清[3H]1,25(OH)2D3蛋白结合研究表明，在37℃。C, 94.8 .+-。0.4%的激素在静脉注射30分钟后蛋白结合。1,25(OH)2D3能够穿过血脑屏障。相对于其血清浓度而言，其有限的脑吸收表明该激素不能自由渗透到中枢神经系统。它的脑摄取可能与血脑屏障中自由循环的1,25(OH)2D3浓度有关。
The brain uptake of [3H]1,25-dihydroxyvitamin D3 ([3H]1,25(OH)2D3) was studied during steady-state conditions using the multiple-indicator dilution technique in dogs. The fractional [3H]1,25(OH)2D3 uptake was evaluated at 0.8 .+-. 0.15% during a single passage through the dog brain. Evaluation of the [3H]1,25(OH)2D3 uptake by the vitamin D-replete and vitamin D-depleted rat brain indicated that 30 min after its injection, the fractional uptake was not influenced by the vitamin D status of the animals or by the amount of [3H]-1,25(OH)2D3 brain accumulation was between 0.16-0.20%; the brain-to-serum ratio varied between 5-6%. Protein-binding studies of serum [3H]1,25(OH)2D3 indicated that, at 37.degree. C, 94.8 .+-. 0.4% of the hormone was protein bound 30 min after its i.v. injection. 1,25(OH)2D3 is able to cross the blood-brain barrier. Its limited brain uptake in relation to its serum concentration suggests that the hormone does not penetrate freely into the CNS. Its brain uptake may be related to the free circulating 1,25(OH)2D3 concentration perfusing the blood-brain barrier.