The microtubule end-binding protein FgEB1 regulates polar growth and fungicide sensitivity via different interactors in Fusarium graminearum
The microtubule end-binding protein FgEB1 regulates polar growth and fungicide sensitivity via different interactors in Fusarium graminearum
复制标题
微管末端结合蛋白 FgEB1 通过不同相互作用因子调节禾谷镰刀菌的极性生长和杀菌剂敏感性
DOI：
10.1111/1462-2920.13651
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发表时间：
2017-05-01
影响因子：
5.1
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Liu, Zunyong;Wu, Sisi;Ma, Zhonghua
在酵母中，末端结合蛋白1（EB1）同源物调节微管动力学、细胞极化和染色体稳定性。然而，EB1直系同源物在植物病原真菌中的功能尚未被表征。在这里，我们观察到FgEB 1缺失突变体（FgEB 1）的禾谷镰刀菌表现出扭曲的菌丝，增加菌丝分支和弯曲的分生孢子，表明FgEB 1参与细胞极性的调节。显微镜检查进一步表明，与野生型相比，FgEB 1的微管表现出更少的组织化。此外，缺乏FgEB 1也改变了分布的极性相关的I类肌球蛋白通过与肌动蛋白的相互作用。另一方面，我们确定了四个核心septins FgEB 1相互作用的蛋白质，并发现FgEB 1和septins调节分生孢子极性生长在相反的方向。有趣的是，FgEB 1和FgKar9构成了另一种复合物，可以调节对多菌灵（一种特异性微管损伤剂）的反应。此外，FgEB 1的缺失导致脱氧雪腐镰刀菌烯醇（DON）的生物合成显着减少。综上所述，本研究的结果表明，FgEB 1通过不同的相互作用物调节细胞极性、杀菌剂敏感性和DON生物合成。graminarum，为了解EB 1在丝状真菌中的生物学功能提供了新的见解。
In yeasts, the end-binding protein 1 (EB1) homologs regulate microtubule dynamics, cell polarization, and chromosome stability. However, functions of EB1 orthologs in plant pathogenic fungi have not been characterized yet. Here, we observed that the FgEB1 deletion mutant (FgEB1) of Fusarium graminearum exhibits twisted hyphae, increased hyphal branching and curved conidia, indicating that FgEB1 is involved in the regulation of cellular polarity. Microscopic examination further showed that the microtubules of FgEB1 exhibited less organized in comparison with those of the wild type. In addition, the lack of FgEB1 also altered the distribution of polarity-related class I myosin via the interaction with the actin. On the other hand, we identified four core septins as FgEB1-interacting proteins, and found that FgEB1 and septins regulated conidial polar growth in the opposite orientation. Interestingly, FgEB1 and FgKar9 constituted another complex that modulated the response to carbendazim, a microtubule-damaging agent specifically. In addition, the deletion of FgEB1 led to dramatically decreased deoxynivalenol (DON) biosynthesis. Taken together, results of this study indicate that FgEB1 regulates cellular polarity, fungicide sensitivity and DON biosynthesis via different interactors in F. graminarum, which provides a novel insight into understanding of the biological functions of EB1 in filamentous fungi.