Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins.
Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins.
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酵母 Ulp1 是一种 Smt3 特异性蛋白酶，与核孔蛋白结合。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Yoshiko Kikuchi
中科院分区：
文献类型：
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作者：
Yoshimitsu Takahashi;Junya Mizoi;Akio Toh;Yoshiko Kikuchi
酵母Smt3是一种类似于哺乳动物相扑-1的泛素样蛋白。Cdc3是一种Septin组分，已知可被Smt3修饰。这种修饰的水平受到Smt3特异性蛋白酶突变ulp1-ts或ULP1过表达的影响。通过双杂交筛选，共分离到5个uIP(Ulp1相互作用蛋白)基因。UIP1与编码核孔复合体(NPC)组成部分的NUP42相同。在双杂交系统和免疫共沉淀实验中，另一个鼻咽癌相关成分Gle1也与Ulp1相互作用。因此，Ulp1与核孔蛋白结合，并可能与末期的Septin环相互作用。
Yeast Smt3 is a ubiquitin-like protein similar to the mammalian SUMO-1. Cdc3, a septin component, is known to be modified by Smt3. The level of this modification was affected by Smt3-specific protease mutation ulp1-ts or overexpression of ULP1. By two-hybrid screening, we isolated 5 UIP (Ulp1 interacting protein) genes. UIP1 was identical to NUP42 encoding a component of the nuclear pore complex (NPC). Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and co-immunoprecipitation experiment. Thus Ulp1 associates with nucleoporins and may interact with septin rings in the telophase.