Vision tests in the mouse: Functional phenotyping with electroretinography
Vision tests in the mouse: Functional phenotyping with electroretinography
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DOI：
10.2741/3409
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发表时间：
2009-01-01
影响因子：
3.1
通讯作者：
Seeliger, Mathias W.
中科院分区：
文献类型：
--
作者：
Tanimoto, Naoyuki;Muehlfriedel, Regine L.;Seeliger, Mathias W.
视网膜电图（ERG）是临床眼科中已建立的诊断技术，并提供有关视网膜功能的客观信息。这项技术也应用于基础研究，其中遗传性视网膜病变的动物模型显著有助于我们理解一般ERG反应的组成以及视网膜退行性病变如何具体改变视网膜功能。事实上，转基因小鼠的电生理评估，这是遗传工程模拟人类突变，导致视网膜疾病，可以很好地与临床数据进行比较。此外，与人类患者相比，在评估小鼠时，对检查的限制（例如测量长度、光强度范围）的关注要少得多。为了正确测量和分析视网膜反应，必须考虑几个重要方面。本文集中在这些方面，并显示了从正常野生型小鼠和具有特定功能特性的转基因小鼠获得的示例性ERG数据，即Rho（-/-）（视杆视蛋白敲除，仅视锥功能）和Cnga 3（-/-）（视锥CNG通道缺陷，仅视杆功能），以说明视杆和视锥系统对ERG响应的贡献。
Electroretinography (ERG) is an established diagnostic technique in clinical ophthalmology and provides objective information about retinal function. This technique is also applied in basic research, where animal models of hereditary retinopathies have significantly contributed to our understanding of the composition of ERG responses in general and how retinal degenerative pathologies alter retinal function specifically. Indeed, electrophysiologic assessment of transgenic mice, which are genetically engineered to mimic human mutations that lead to retinal diseases, can be well compared with clinical data. Furthermore, limitations on examinations (e.g. length of measurement, range of light intensity) are much less of a concern when assessing mice compared to human patients. In order to measure and analyze retinal responses properly, several important aspects have to be considered. This paper focuses on these aspects, and shows exemplary ERG data which were obtained from normal wild-type mice and from transgenic mice with specific functional properties, namely Rho(-/-) (rod opsin knockout, cone function only), and Cnga3(-/-)(cone CNG channel deficient, rod function only) to illustrate rod and cone system contributions to ERG responses.