LIVER PROTEIN-SYNTHESIS - MOLECULAR-WEIGHT DISTRIBUTION OF PULSE-LABELED POLYPEPTIDE-CHAINS IN NORMAL AND THYROIDECTOMIZED RATS
LIVER PROTEIN-SYNTHESIS - MOLECULAR-WEIGHT DISTRIBUTION OF PULSE-LABELED POLYPEPTIDE-CHAINS IN NORMAL AND THYROIDECTOMIZED RATS
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DOI：
10.1016/0005-2787(76)90028-9
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
HASCHEMEYER, AEV
中科院分区：
文献类型：
--
作者：
MATHEWS, RW;HASCHEMEYER, AEV
提出了用于测定多肽链分子量的异质群体的体内延伸率的方程。本文用十二烷基硫酸钠凝胶电泳法测定了大鼠肝脱氧胆酸盐可溶性蛋白质中脉冲标记多肽链的分布，并计算了一条理论曲线，用于测定分子量为50，000的多肽链的合成时间（tc_（50））。正常和切除甲状旁腺的Long-Evans雄性大鼠的Tc 50值分别为1.2和1.75 min，代表蛋白质合成速率约为7.5和5.1 mg蛋白质/g肝脏/h。在标记或酰胺黑染色的基础上，未观察到两组之间肝脏多肽链的分子量谱存在差异。比较了标记血浆蛋白分泌前后的放射性分布。蛋白质合成率的变化与甲状腺激素相关的酶水平的作用进行了讨论。
Equations are presented for determination of elongation rate in vivo for a heterogeneous population of polypeptide chain molecular weights. The distribution of pulse-labeled polypeptide chains in rat liver deoxycholate-soluble protein was obtained by sodium dodecyl sulfate-gel electrophoresis an used to compute a theoretical curve for determination of synthesis time of a 50,000 molecular weight polypeptide chain (tc50). Values of Tc50 for normal and thyroparathyroidectomized Long-Evans male rats were 1.2 and 1.75 min, respectively, representing protein synthetic rates of about 7.5 and 5.1 mg protein/g liver per h. No difference in the molecular weight profile of liver polypeptide chains on the basis of labeling or Amido-black staining was observed between the 2 groups. The distributions of radioactivity before and after secretion of labeled plasma protein are compared. The role of protein-synthetic rate in the changing enzyme levels associated with thyroid hormone is discussed.