Effects of n-6 polyunsaturated fatty acids on prostaglandin production in ovine fetal chorion cells in vitro in late gestation ewes
Effects of n-6 polyunsaturated fatty acids on prostaglandin production in ovine fetal chorion cells in vitro in late gestation ewes
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DOI：
10.1016/j.placenta.2011.06.026
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发表时间：
2011-10-01
期刊：
影响因子：
3.8
通讯作者：
Wathes, D. C.
中科院分区：
文献类型：
--
作者：
Cheng, Z.;Elmes, M.;Wathes, D. C.
目的：建立羊胎盘体外模型，研究补充n-6多不饱和脂肪酸（PUFA）对前列腺素（PG）生成的影响。PGs是胎儿成熟和分娩的关键调节因子。研究设计：在妊娠晚期（第135天）收集胎儿尿囊绒毛膜组织（FC）。在无血清培养基中，用0 ~ 100 μ M亚油酸（LA）、γ -亚麻酸（GLA）或花生四烯酸（AA）培养FC细胞，并用对照培养基、脂多糖（LPS, 0.1 μ g/ml）、地塞米松（DEX, 5 μ M）或LPS （0.1 μ g/ml）与DEX （5 μ M）联合激发。在挑战后2 h和24 h收获废培养基，用于测量pg。主要观察指标：评估治疗对胎盘1系和2系PGE生成的影响。结果：补充LA抑制PGE(1)和PGE(2)的产生。GLA主要促进了PGE(1)的生成，尽管它也增加了PGE(2)的产量。补充AA显著增加了PGE(2)的产生，但也刺激了PGE(1)。DEX加LPS或不加LPS均抑制PG生成。补充n-6 PUFAs可以减弱或中和LPS对FC细胞产生PGE(1)和PGE(2)的刺激作用。结论：这些数据表明，补充n-6 PUFAs会改变胎盘PG的生成，但其确切作用取决于它们在PG合成生物合成途径中的位置。这项研究支持了一种可能性，即含有GLA的油，被广泛推广为膳食补充剂，可能通过抑制胎盘中PGE(2)产生对LPS挑战的反应来降低早产风险。(C) 2011 Elsevier Ltd.版权所有。
Objective: To use an in vitro model of the ovine placenta to determine effects of n-6 polyunsaturated fatty acid (PUFA) supplementation on prostaglandin (PG) production. PGs are key regulators of fetal maturation and parturition.Study design: Fetal allantochorion tissue (FC) was collected in late pregnancy (day 135). FC cells were isolated and cultured with 0-100 mu M of linoleic acid (LA), gamma-linolenic acid (GLA) or arachidonic acid (AA) in serum free medium and challenged with control medium, lipopolysaccharide (LPS, 0.1 mu g/ml), dexamethasone (DEX, 5 mu M) or a combination of LPS (0.1 mu g/ml) with DEX (5 mu M). Spent medium was harvested at 2 h and 24 h post challenge for measuring PGs.Main outcome measures: To assess the effects of treatment on placental 1- and 2-series PGE production.Results: LA supplementation inhibited both PGE(1) and PGE(2) production. GLA predominantly stimulated PGE(1) generation, although it also increased PGE(2) production. AA supplementation predominantly increased PGE(2) production, but also stimulated PGE(1). DEX treatment with or without LPS inhibited PG production. Supplementation with n-6 PUFAs attenuated or neutralised the stimulatory effect of LPS challenge on FC cells for both PGE(1) and PGE(2) production.Conclusion: These data show that supplementation with n-6 PUFAs alters placental PG production, but their precise effects depend on their position in the biosynthetic pathway for PG synthesis. This study supports the possibility that GLA containing oils, widely promoted as dietary supplements, might reduce the risk of pre-term labour by inhibiting the responsiveness of PGE(2) production to LPS challenge in the placenta. (C) 2011 Elsevier Ltd. All rights reserved.