Genomewide demarcation of RNA polymerase II transcription units revealed by physical fractionation of chromatin
Genomewide demarcation of RNA polymerase II transcription units revealed by physical fractionation of chromatin
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DOI：
10.1073/pnas.1131966100
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发表时间：
2003-05-27
影响因子：
11.1
通讯作者：
Lieb, JD
中科院分区：
文献类型：
--
作者：
Nagy, PL;Cleary, ML;Lieb, JD
染色质的表观遗传修饰在调节基因组DNA的表达和可及性中起重要作用。在这里，我们报告了一个全基因组的方法，用于将酵母染色质分成两个功能不同的部分，一个包含RNA聚合酶II转录序列，另一个包含非编码序列和RNA聚合酶I和III转录的基因。非编码区可以进一步分为启动子和缺乏启动子的片段。观察到的分离显然是基于不同基因组区域中染色质的差异交联效率。结果揭示了一个全基因组的分子机制，用于标记启动子和基因组区域，这些区域有被RNA聚合酶II转录的许可证，这是一种以前未被认识到的基因组复杂性水平，可能存在于所有真核生物中。我们的方法具有广泛的潜在用途，作为一种工具，基因组注释和表征全球变化的染色质结构，伴随着不同的遗传，环境和疾病状态。
Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the other comprising noncoding sequences and genes transcribed by RNA polymerases I and III. Noncoding regions could be further fractionated into promoters and segments lacking promoters. The observed separations were apparently based on differential crosslinking efficiency of chromatin in different genomic regions. The results reveal a genomewide molecular mechanism for marking promoters and genomic regions that have a license to be transcribed by RNA polymerase II, a previously unrecognized level of genomic complexity that may exist in all eukaryotes. Our approach has broad potential use as a tool for genome annotation and for the characterization of global changes in chromatin structure that accompany different genetic, environmental, and disease states.