Soluble Fiber Viscosity Affects Both Goblet Cell Number and Small Intestine Mucin Secretion in Rats
Soluble Fiber Viscosity Affects Both Goblet Cell Number and Small Intestine Mucin Secretion in Rats
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DOI：
10.3945/jn.109.110171
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发表时间：
2009-09-01
影响因子：
4.2
通讯作者：
Morita, Tatsuya
中科院分区：
文献类型：
--
作者：
Ito, Hiroyuki;Satsukawa, Mitsuru;Morita, Tatsuya
我们研究了可溶性纤维粘度在小肠粘蛋白分泌中的作用。粘度定义为粘度曲线下面积(VAUC)。分别以50g/kg饲喂对照饲料或添加魔芋甘露聚糖(KM)[低、中、高分子量(LKM、MKM、HKM)]饲料10d，腔粘蛋白含量和杯状细胞数随KM分子量的增加而增加。同时摄入2克纤维素酶/公斤饲料，对HKM饮食的这种影响被抵消。饲喂含LKM、MKM、HKM、瓜尔豆胶(HGG、LGG)、木耳(PS)或果胶(PC)50g/kg的饲料。VAUC较高的纤维(MKM、HKM、HGG和PS)增加杯状细胞的数量，但VAUC较低的纤维(LKM、LGG和PC)则不增加杯状细胞的数量。在喂食HKM、PC和PS的大鼠中，管腔粘蛋白含量更高。杯状细胞数与VAUC呈正相关(r=0.98；P<；0.01)。在饲喂HKM饲料的大鼠中，回肠Muc2基因的表达没有受到影响，但Muc3基因的表达低于对照组，这表明摄入HKM饲料后肠腔粘蛋白的增加与MUC基因表达的增强无关。5‘-溴脱氧尿嘧啶核苷(BrdU)掺入研究表明，喂食HKM饲料的大鼠绒毛上BrdU标记细胞的位置高于喂饲对照饲料的大鼠。结果表明，除PC纤维外，可溶性纤维通过增加杯状细胞数量与纤维VAUC成比例地上调管腔粘蛋白的基线分泌。J.Nutr.139：1640-1647,2009。
We examined the role of soluble fiber viscosity in small intestinal mucin secretion. Viscosities were defined as the area under the viscosity curve (VAUC). Rats were fed a control diet or diets containing konjac mannan (KM) [low, medium, or high molecular weight (LKM, MKM, HKM), respectively] at 50 g/kg diet for 10 d. Luminal mucin content and goblet cell number increased in proportion to the molecular weight of KM. Such effects with the HKM diet were nullified by the concurrent ingestion of 2 g cellulase/kg diet. Diet containing LKM, MKM, HKM, guar gums (high or low molecular weight; HGG, LGG), psyllium (PS), or pectin (PC) at 50 g/kg was fed to rats. Fibers with higher VAUC (MKM, HKM, HGG, and PS) increased goblet cell numbers, but not those with lower VAUC (LKM, LGG, and PC). Luminal mucins were greater in rats fed HKM, PC, and PS diets. Goblet cell numbers and VAUC were correlated (r = 0.98; P < 0.01). In rats fed the HKM diet, ileal Muc2 gene expression was not affected, but that of Muc3 was lower than in those fed the control diet, indicating that the increase in luminal mucins after ingestion of HKM diet occurred independently of enhanced Muc gene expression. An incorporation study of 5'-bromo-deoxyuridine (BrdU) showed the position of the uppermost-BrdU labeled cell along the villi was higher in rats fed the HKM diet than in those fed the control diet. The results suggest that soluble fibers, except PC, upregulate baseline secretion of luminal mucins by increasing goblet cell numbers in proportion to fiber VAUC. J. Nutr. 139:1640-1647, 2009.