In vivo carbon-13 nuclear magnetic resonance studies of heart metabolism.
In vivo carbon-13 nuclear magnetic resonance studies of heart metabolism.
复制标题
心脏代谢的体内碳13核磁共振研究。
DOI：
10.1073/pnas.80.6.1603
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Shulman,RG
中科院分区：
文献类型：
--
作者：
Neurohr,KJ;Barrett,EJ;Shulman,RG
用~（13）C NMR（20.18）MHz研究了豚鼠心脏在体代谢。高品质的质子去耦13 C NMR光谱具有良好的信噪比和分辨率，可以在6分钟内获得。自然丰度谱显示共振，可以分配给脂肪酸，但糖原没有看到。在静脉输注D-[1- 13 C]葡萄糖和胰岛素期间，连续跟踪心肌糖原合成的时间过程长达4小时，缺氧导致标记的糖原在6分钟内降解，并在乳酸中出现13 C标记。输注[2- 13 C]乙酸钠导致标记掺入谷氨酸和谷氨酰胺的C-4、C-2和C-3位置，反映了三羧酸循环活性预期的标记“混乱”。对豚鼠体内心脏的31 P NMR光谱的检查表明，在13 C NMR实验的时间段内，高能磷酸盐没有变化。我们的研究表明，13 C NMR是一种独特的非破坏性的工具，在体内心脏代谢的研究。
Guinea pig heart metabolism was studied in vivo by 13C NMR at 20.18 MHz. High-quality proton-decoupled 13C NMR spectra with excellent signal-to-noise ratios and resolution could be obtained in 6 min. Natural-abundance spectra showed resonances that could be assigned to fatty acids, but glycogen was not seen. During intravenous infusion of D-[1-13C]glucose and insulin, the time course of myocardial glycogen synthesis was followed serially for up to 4 hr. Anoxia resulted in degradation of the labeled glycogen within 6 min and appearance of 13C label in lactic acid. Infusion of sodium [2-13C]acetate resulted in incorporation of label into the C-4, C-2, and C-3 positions of glutamate and glutamine, reflecting "scrambling" of the label expected from tricarboxylic acid cycle activity. Examination of the 31P NMR spectrum of the guinea pig heart in vivo demonstrated no change in the high-energy phosphates during the time periods of the 13C NMR experiments. Our studies indicate that 13C NMR is a unique non-destructive tool for the study of heart metabolism in vivo.