Defervescent dengue patients might be a potential source of infection for vector mosquitoes
Defervescent dengue patients might be a potential source of infection for vector mosquitoes
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DOI：
10.1186/s40249-020-0631-8
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发表时间：
2020-03-02
影响因子：
8.1
通讯作者：
Chen, Xiao-Guang
中科院分区：
文献类型：
--
作者：
Xu, Ye;Zhao, Ling-Zhai;Chen, Xiao-Guang
登革热是一种重新出现的公共卫生问题和蚊媒传染病，主要由埃及伊蚊和伊蚊传播。蚊。早期诊断、隔离和治疗患者是控制登革热流行的关键步骤，特别是防止登革热病毒（DENV）的继发性传播。然而，人们对发热登革热患者作为感染源知之甚少。方法对2013年6月至2014年12月广州市第八人民医院收治的1268例登革热患者进行病例分析。使用实时逆转录聚合酶链反应测量每个个体的病毒载量。Ae。埃及伊蚊和伊蚊。研究白纹伊蚊与登革热病毒载量分级的血餐接触，探讨登革热患者病毒血症与病媒蚊传播能力的关系。结果发热后第1天至第12天血中病毒数在10(8)~ 10(3)拷贝/ml之间。媒介能力分析；埃及伊蚊和伊蚊。白纹伊蚊检测到病毒血症bbb10(4)拷贝/ml仍可感染媒介蚊，提示发热登革热患者可能是感染源之一。结论部分发热登革热患者仍有足够的病毒载量感染媒介蚊。因此，应扩大对这些人的蚊虫叮咬保护，以防止发生二次传播事件。
BackgroundDengue is a re-emerging public health problem and mosquito-borne infectious disease that is transmitted mainly by Aedes aegypti and Ae. albopictus. Early diagnosis, isolation, and treatment of patients are critical steps for dengue epidemic control, especially to prevent secondary transmission of dengue virus (DENV). However, little is known about defervescent dengue patients as a source of infection.MethodsThis case study describes 1268 dengue patients hospitalized at Guangzhou Eighth People's Hospital from June 2013 to December 2014. The viral loads of each individual were measured using real-time reverse transcription-polymerase chain reaction. Ae. aegypti and Ae. albopictus were exposed to blood meal with gradated dengue viral loads to characterize the relationship between viremia in dengue patients and the vector competence of vector mosquitoes.ResultsThe viral numbers in the blood were measured, ranging from 10(8) to 10(3) copies/ml from day 1 to day 12 after fever onset. Vector competence analysis of Ae. aegypti and Ae. albopictus indicated that viremia >10(4) copies/ml can still infect vector mosquitoes, which implied that the defervescent dengue patients might be a source of infection.ConclusionsThe results of this study indicate that some defervescent dengue patients still have sufficient viral load to infect vector mosquitoes. Therefore, the protection against mosquito biting for these people should be extended to prevent secondary transmission events.