The involvement of heat shock protein and cytochrome P450 genes in response to UV-A exposure in the beetle Tribolium castaneum
The involvement of heat shock protein and cytochrome P450 genes in response to UV-A exposure in the beetle Tribolium castaneum
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赤拟谷盗甲虫中热休克蛋白和细胞色素 P450 基因对 UV-A 暴露的反应
DOI：
10.1016/j.jinsphys.2012.03.007
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发表时间：
2012-06-01
影响因子：
2.2
通讯作者：
Lei, Chao-Liang
中科院分区：
文献类型：
--
作者：
Sang, Wen;Ma, Wei-Hua;Lei, Chao-Liang
阳光是影响地球上所有生物的重要环境因素。紫外线A （UV-A）是在阳光中发现的众多频段之一。许多动物利用UV-A来获得视觉线索，例如在觅食和择偶时。然而，UV-A也会引起损伤，如氧化应激、DNA损伤和细胞凋亡。在本研究中，我们研究了UV-A对红粉甲虫成虫热休克蛋白（Hsp）和细胞色素CYP6BQ家族几个应激响应基因的存活、繁殖力和表达谱的影响。结果表明：短期UV-A照射（365 nm, < 4 h）对甲虫的存活和繁殖力没有影响；然而，Hsp27、Hsp68、Hsp83、CYP6BQ4和CYP6BQ8 mRNA水平在UV-A暴露的前2小时显著升高。其中，Hsp68上调幅度最大，增加了8.9倍。这些结果表明，这些基因可能参与防御有害的UV-A辐射。此外，我们还研究了Hsp和P450基因家族以类似模式诱导的基因启动子序列中的潜在转录因子结合基序（TFBMs）；结果表明，这些基序与哺乳动物的环境应激转录因子结合位点高度同源。我们的实验表明，UV-A照射可以影响具有通用tfbm的应激反应基因（如Hsps和p450）的表达谱，这些基因可能参与减少照射带来的生态挑战。(C) 2012 Elsevier Ltd.版权所有。
Sunlight is an important environmental factor that affects all living organisms on Earth. Ultraviolet A (UV-A) is one of the many frequency bands found in sunlight. Many animals use UV-A to attain visual cues, for example, in foraging and mate selection. However, UV-A can also induce damage, such as oxidative stress, DNA lesions and apoptosis. In the present study, we investigated the effects of UV-A on the survival, fecundity and expression profiles of several stress-responsive genes belonging to the heat shock protein (Hsp) and the cytochrome CYP6BQ families from the adult red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The results showed that short-term UV-A exposure (365 nm, < 4 h) did not influence the survival or fecundity of the beetles; however, Hsp27, Hsp68, Hsp83, CYP6BQ4 and CYP6BQ8 mRNA levels significantly increased during the first 2 h of UV-A exposure. Among them, Hsp68 was the most highly up-regulated, increasing by 8.9-fold. These results indicate that these genes may participate in the defense against harmful UV-A radiation. In addition, we investigated the potential transcription factor binding motifs (TFBMs) in the promoter sequences of genes induced in similar pattern from the Hsp and P450 gene families; the results indicated that, these motifs are highly homologous to environmental stress transcription factor binding sites in mammals. Our experiments revealed that UV-A irradiation could influence the expression profile of stress-responsive genes, such as Hsps and P450s, which have universal TFBMs, and that these genes may be involved in reducing the ecological challenges posed by irradiation. (C) 2012 Elsevier Ltd. All rights reserved.