Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots
Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots
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DOI：
10.1007/s11240-009-9568-0
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Triplett, Barbara A.
中科院分区：
文献类型：
--
作者：
Frankfater, Cheryl R.;Dowd, Michael K.;Triplett, Barbara A.
研究了水杨酸和茉莉酸甲酯两种化学诱导剂对海岛棉毛状根中棉酚、6-甲氧基棉酚和6，6 ′-二甲氧基棉酚合成的影响。茉莉酸甲酯，而不是水杨酸，被发现增加棉酚及其甲基化形式的生产，但伴随着减少培养物的生长。茉莉酸甲酯的最佳剂量为100和300 μ M之间的毛状根收获后7天的启发。用100 μ M茉莉酸甲酯诱导20天后，棉酚水平增加了8倍，观察到在引发的文化与对照文化相比，两倍的最高棉酚水平以前报道的任何棉花组织。还观察到6-甲氧基棉酚水平增加2至3倍，6，6 '-二甲氧基棉酚水平略有增加。虽然茉莉酸甲酯刺激棉酚的两种光学形式的生产，对映体的分布是不同的引发和控制文化之间。
The effect of two chemical elicitors, salicylic acid and methyl jasmonate, on the production of gossypol, 6-methoxygossypol, and 6,6'-dimethoxygossypol in Gossypium barbadense hairy roots was examined. Methyl jasmonate, but not salicylic acid, was found to increase the production of gossypol and its methylated forms, but with a concomitant reduction in culture growth. The optimal methyl jasmonate dose was between 100 and 300 mu M for hairy roots harvested 7 days after elicitation. After 20 d of induction with 100 mu M methyl jasmonate, an eightfold increase in the level of gossypol was observed in elicited cultures compared with control cultures, double the highest gossypol levels previously reported for any cotton tissue. A two to threefold increase in the level of 6-methoxygossypol and a slight increase in the levels of 6,6'-dimethoxygossypol were also observed. Although methyl jasmonate stimulated the production of both optical forms of gossypol, the distribution of the enantiomers was different between elicited and control cultures.