OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice
OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice
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DOI：
10.1007/s00425-011-1532-7
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发表时间：
2012-04-01
期刊：
影响因子：
4.3
通讯作者：
Wu, Changyin
中科院分区：
文献类型：
--
作者：
Gao, Xinqiang;Chen, Zhihui;Wu, Changyin
虽然编码WD 40基序的大量基因已被鉴定为参与拟南芥的各种发育过程，但对这些基因在水稻（Oryza sativa）中的功能知之甚少。在这里，我们报告了一个新的水稻基因OsLIS-L1（Lissencephaly type-1-like 1）的克隆和功能特性，这是正常生育和第一节间伸长所需的。OsLIS-L1基因编码一种类似无脑畸形1型的蛋白质，该蛋白质含有人类大脑发育所需的WD 40基序。SMART算法分析表明，OsLIS-L1含有一个LIS 1同源结构域（LisH）、一个C端与LisH同源的结构域（CTLH）、一个位于中间的5个WD 40重复结构域和一个与三聚体G蛋白β亚基（G（beta））同源的4个WD 40重复结构域。OsLIS-L1基因在茎和穗中的表达量相对较高，在穗的不同发育阶段具有动态表达模式。两个独立的等位基因，指定oslis-l1-1和oslis-l1-2，表现出类似的异常发育表型，包括半矮秆，短穗长度，降低雄性育性。细胞学检测证实OsLIS-L1不影响花粉母细胞减数分裂。与野生型相比，oslis-l1突变体雄配子体形成异常，但花药壁和花粉壁发育不受影响。组织学分析表明，OsLIS-L1基因调控穗下第一节间细胞的增殖。结果表明，OsLIS-L1基因在水稻雄配子体形成和第一节间伸长过程中起重要作用。
Although a large number of genes encoding the WD40 motif have been identified as being involved in various developmental processes in Arabidopsis, little is known about the function of these genes in rice (Oryza sativa). Here, we report the cloning and functional characterization of a novel rice gene OsLIS-L1 (Lissencephaly type-1-like 1), which is required for normal fertility and the first internode elongation. OsLIS-L1 encodes a lissencephaly type-1-like protein containing the WD40 motif that is required for brain development in human. SMART algorithm analysis indicated that OsLIS-L1 contains a LIS1 homology (LisH) domain, a C terminus to LisH (CTLH) domain, a five WD40-repeat domain in the middle, and a domain with four WD40 repeats which is homologous to the beta subunit of trimeric G-proteins (G(beta)). OsLIS-L1 transcript is relatively highly abundant in stem and panicle and has a dynamic expression pattern at different panicle developmental stages. Two independent alleles, designated oslis-l1-1 and oslis-l1-2, exhibited similar abnormal developmental phenotypes, including semi-dwarf, shorter panicle length, and reduced male fertility. Cytological examination confirmed that OsLIS-L1 does not affect the meiosis in pollen mother cells. Compared with wild type, the oslis-l1 mutant had abnormal male gametophyte formation, but anther cell wall and pollen wall development were not affected. Histological analysis revealed that OsLIS-L1 regulates the cell proliferation in the first internode under the panicle. Our results indicate that OsLIS-L1 plays an important role in male gametophyte formation and the first internode elongation in rice.