Spread of varicella-zoster virus DNA to family members and environments from siblings with varicella in a household
Spread of varicella-zoster virus DNA to family members and environments from siblings with varicella in a household
复制标题
DOI：
10.1542/peds.103.5.e61
复制
发表时间：
1999-05-01
期刊：
影响因子：
8
通讯作者：
Suga, S
中科院分区：
文献类型：
--
作者：
Asano, Y;Yoshikawa, T;Suga, S
Objective.为了阐明水痘病毒从兄弟姐妹传播到其他家庭成员和环境对象在一个家庭设置之前和之后的疾病。材料和方法。在一个由五名成员组成的家庭中，一名男孩患上了水痘，其余两名兄弟姐妹在指数病例发病后17和18天患上了这种疾病。在疾病发作之前和/或之后，经常收集父母和三个兄弟姐妹的喉咙和手的拭子样本以及来自房屋环境中几个来源的样本。采用敏感的聚合酶链反应扩增法检测标本中水痘-带状疱疹病毒（VZV）DNA。总共从三名水痘患儿的喉咙和手上采集了108份样本，从父母那里采集了72份样本，从家中几个来源的表面采集了72份样本。在指示病例（大男孩）发病后8天，在指示病例的两个样本和四个来源（空调过滤器，桌子，电视频道按钮和门把手）的表面上检测到VZV DNA，但不是来自其他两个兄弟姐妹或父母。然后，到1998年1月30日，在母亲的手上和空调过滤器上检测到一次，在电视频道按钮上检测到三次，当时该女孩在发病后17天患上水痘。弟弟于1月31日发病。在1月30日获得的任何样本中都没有检测到病毒DNA;然而，1月31日在大男孩和父亲的手上以及女孩的手皮喉咙样本中检测到了病毒DNA。此后，至2月13日，在手上间歇性检测到3次病毒DNA，在女童喉咙持续检测到3次病毒DNA。在2月1日至3日期间，在2月1日至4日期间，在年龄较小的男孩的喉咙中，在手部皮肤上检测到三次病毒DNA。2月2日至13日期间，在大男孩及其父母的手上偶尔检出，在4种环境源表面偶尔或间歇检出。目前的研究表明，当水痘患者出现在一个家庭中时，VZV DNA会迅速和广泛地污染环境，尽管这并不直接意味着传染性。
Objective. To elucidate virus spread from siblings with varicella to other family members and environmental objects in a family setting before and after onset of the disease.Materials and Methods. Among a family consisting of five members, a boy developed varicella and the remaining two siblings developed the disease 17 and 18 days after onset of the index case. Swab samples from throats and hands of parents and three siblings and samples from several sources in the environment of the house were collected frequently before and/or after onset of the disease. Varicella-zoster virus (VZV) DNA in the samples was examined by a sensitive polymerase chain reaction amplification assay.Results. In total, 108 samples from the throats and hands of the three children with varicella, 72 such samples from parents, and 72 samples from the surfaces of several sources in the house were collected. Eight days after onset of the index case (the older boy), VZV DNA was detected in both samples from the index case and on the surfaces of four sources (air conditioner filter, table, television channel push-buttons, and door handle), but not from the two other siblings or parents. Then, it was detected once on the mother's hand and the air conditioner filter and three times on the television channel push-buttons by January 30, 1998, when the girl developed varicella, 17 days after onset of the index case. The younger brother developed the disease on January 31. Viral DNA could not be detected in any samples obtained on January 30; however, it was detected on the hands of the older boy and the father and in samples from the hand rind throat of the girl on January 31. Thereafter, virus DNA was detected three times intermittently by February 13 on the hand and three times persistently in the throat of the girl. The virus DNA was detected three times between February 1 and 3 on the hand rind three times between February 1 and 4 in the throat of the younger boy. It was detected occasionally on the hands of the older boy and the parents, and occasionally or intermittently on surfaces of four environmental sources between February 2 and 13.Conclusions. The present study showed the rapid and broad contamination of the environment with the VZV DNA when the varicella patient appeared in a family, although it does not directly mean infectivity.