MipZ, a spatial regulator coordinating chromosome segregation with cell division in Caulobacter
MipZ, a spatial regulator coordinating chromosome segregation with cell division in Caulobacter
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DOI：
10.1016/j.cell.2006.05.038
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发表时间：
2006-07-14
期刊：
影响因子：
64.5
通讯作者：
Shapiro, Lucy
中科院分区：
文献类型：
--
作者：
Thanbichler, Martin;Shapiro, Lucy
分裂面的正确定位是产生具有正常染色体互补的子细胞的先决条件。在这里，我们提出了一种机制，协调组装和放置的FtsZ细胞动力学环与双极本地化的新复制的染色体起源柄杆菌。在位于极性的起始区复制后，一个拷贝以MreB依赖性方式快速移动到细胞的另一端。一个以前未知的基本蛋白质，MipZ，形成一个复杂的分配蛋白ParB附近的复制起点和本地化的复制的起源地区的细胞两极。MipZ直接干扰FtsZ聚合，从而将FtsZ环形成限制在中间细胞，即MipZ浓度最低的区域。因此，MipZ的细胞定位具有定位FtsZ环和延迟细胞分裂器形成的双重功能，直到染色体分离开始。
Correct positioning of the division plane is a prerequisite for the generation of daughter cells with a normal chromosome complement. Here, we present a mechanism that coordinates assembly and placement of the FtsZ cytokinetic ring with bipolar localization of the newly duplicated chromosomal origins in Caulobacter. After replication of the polarly located origin region, one copy moves rapidly to the opposite end of the cell in an MreB-dependent manner. A previously uncharacterized essential protein, MipZ, forms a complex with the partitioning protein ParB near the origin of replication and localizes with the duplicated origin regions to the cell poles. MipZ directly interferes with FtsZ polymerization, thereby restricting FtsZ ring formation to midcell, the region of lowest MipZ concentration. The cellular localization of MipZ thus serves the dual function of positioning the FtsZ ring and delaying formation of the cell division apparatus until chromosome segregation has initiated.