A biomimetic DNA-based channel for the ligand-controlled transport of charged molecular cargo across a biological membrane
A biomimetic DNA-based channel for the ligand-controlled transport of charged molecular cargo across a biological membrane
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DOI：
10.1038/nnano.2015.279
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发表时间：
2016-02-01
影响因子：
38.3
通讯作者：
Howorka, Stefan
中科院分区：
文献类型：
--
作者：
Burns, Jonathan R.;Seifert, Astrid;Howorka, Stefan
生物离子通道是控制跨细胞膜转运的分子守门人。重建这种系统的功能原理并将其扩展到生理离子负荷之外，这在科学上令人兴奋，并且在技术上与传感或药物释放相关（1，2）。然而，制造具有可预测结构的合成通道仍然是一个重大挑战。在这里，我们使用DNA作为构建材料（4-8）来创建一个原子决定的分子阀，可以控制何时以及哪些货物通过双层运输。这种阀门由七条串联的DNA链组成，可以结合一种特定的配体，作为回应，它会发生纳米机械变化，打开跨膜通道。它还能够以高选择性区分不同的有机小分子的传输，所述有机小分子的不同在于带正电荷或带负电荷的基团的存在。DNA装置可用于控制药物释放和构建合成细胞样或逻辑离子网络（9，10）。
Biological ion channels are molecular gatekeepers that control transport across cell membranes. Recreating the functional principle of such systems and extending it beyond physiological ionic cargo is both scientifically exciting and technologically relevant to sensing or drug release(1,2). However, fabricating synthetic channels" with a predictable structure remains a significant challenge. Here, we use DNA as a building material(4-8) to create an atomistically determined molecular valve that can control when and which cargo is transported across a bilayer. The valve, which is made from seven concatenated DNA strands, can bind a specific ligand and, in response, undergo a nanomechanical change to open up the membrane -spanning channel. It is also able to distinguish with high selectivity the transport of small organic molecules that differ by the presence of a positively or negatively charged group. The DNA device could be used for controlled drug release and the building of synthetic cell-like or logic ionic networks(9,10).