Monodisperse Cell-Encapsulating Peptide Microgel Beads for 3D Cell Culture
Monodisperse Cell-Encapsulating Peptide Microgel Beads for 3D Cell Culture
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DOI：
10.1021/la902827y
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发表时间：
2010-02-16
期刊：
影响因子：
3.9
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Tsuda, Yukiko;Morimoto, Yuya;Takeuchi, Shoji
本文描述了一种用于三维（3D）细胞培养的由自组装肽凝胶组成的单分散细胞包封微凝胶珠的制备方法。我们使用了一个三维微流控轴对称现在聚焦装置与所有外部凝胶化方法。细粉化的盐分散到连续相中，并且当与肽溶液接触时，盐诱导凝胶化。包封后93%以上的细胞存活，细胞在凝胶内迁移和生长。我们的细胞包封珠的应用包括药物测试和工程组织构建中基于珠的细胞测定。
This paper describes a method to produce monodisperse cell-encapsulating microgel beads composed of a self-assembling peptide gel for three-dimensional (3D) cell culture. We used a 3D microfluidic axisymmetric now-focusing device with ail external gelation method. The finely powdered salts were dispersed into a continuous phase, and the Salts induced the gelation when in contact with the peptide solution. Over 93% of the cells survived after the encapsulation, and (he cells migrated and grew Within the gels. Applications of Our cell-encapsulating beads include bead-based cell assays in drug testing and engineering tissue constructs.